Thesis Abstract

Abstract
MicroRNAs (miRNAs) are small non-coding RNA molecules that play crucial roles in the regulation of gene expression at the post-transcriptional level. Dysregulation of miRNA expression has been implicated in various diseases, including cancer. In this thesis, we investigate the role of miRNAs in regulating gene expression in cancer cells, with a focus on understanding the mechanisms by which miRNAs contribute to cancer development and progression. Chapter One provides an introduction to the study, presenting the background of the research, the problem statement, objectives of the study, limitations, scope, significance, structure of the thesis, and definitions of key terms. The literature review in Chapter Two explores existing research on miRNAs and their involvement in cancer, highlighting the importance of understanding the regulatory functions of miRNAs in cancer cells. Chapter Three details the research methodology employed in this study, including sample collection, RNA extraction, miRNA profiling, bioinformatics analysis, and validation of findings. The discussion of findings in Chapter Four presents the results of the study, including the identification of specific miRNAs involved in regulating gene expression in cancer cells and the potential implications for cancer therapy. In conclusion, Chapter Five summarizes the key findings of the study and discusses the broader implications of the research. By investigating the role of miRNAs in regulating gene expression in cancer cells, this thesis contributes to our understanding of the molecular mechanisms underlying cancer development and provides insights that may lead to the development of novel therapeutic strategies targeting miRNAs in cancer treatment. Keywords microRNAs, gene expression, cancer cells, regulatory mechanisms, dysregulation, therapeutic targets.

Thesis Overview

The project titled "Investigating the role of microRNAs in regulating gene expression in cancer cells" aims to explore the intricate mechanisms by which microRNAs modulate gene expression in cancer cells. Cancer is a complex disease characterized by uncontrolled cell growth and proliferation, often driven by genetic mutations and dysregulation of gene expression. MicroRNAs, small non-coding RNA molecules, have emerged as key players in post-transcriptional gene regulation and are known to be dysregulated in various cancer types. The research will delve into the specific microRNAs implicated in cancer progression and investigate how they interact with target genes to influence cancer cell behavior. By elucidating the regulatory networks controlled by microRNAs, this study seeks to uncover novel insights into the molecular pathways underlying cancer development and progression. Through a combination of experimental approaches, including cell culture studies, molecular biology techniques, and bioinformatics analyses, the project aims to identify key microRNAs involved in regulating gene expression in cancer cells. By characterizing the functional roles of these microRNAs and their target genes, the research aims to provide a comprehensive understanding of how microRNAs contribute to the pathogenesis of cancer. Furthermore, the project will explore the potential therapeutic implications of targeting microRNAs for cancer treatment. By identifying specific microRNAs that drive cancer progression, this study may pave the way for the development of novel therapeutic strategies aimed at modulating microRNA activity to inhibit tumor growth and metastasis. Overall, this research overview underscores the significance of investigating the role of microRNAs in regulating gene expression in cancer cells. By shedding light on the molecular mechanisms underlying cancer pathogenesis, this study has the potential to advance our understanding of cancer biology and contribute to the development of targeted therapeutic interventions for cancer patients.