Thesis Abstract

Abstract
This thesis investigates the role of microRNAs in regulating gene expression in cancer cells, with a focus on understanding the underlying mechanisms and potential implications for cancer treatment. The study aims to provide insights into how microRNAs can influence gene expression in cancer cells and how this knowledge can be leveraged for developing novel therapeutic strategies. The introduction section provides an overview of the significance of microRNAs in cancer biology and highlights the need to explore their regulatory functions in gene expression. The background of the study delves into the molecular mechanisms of microRNA-mediated gene regulation and its relevance to cancer development and progression. The problem statement underscores the gaps in understanding the specific roles of microRNAs in cancer cells and the potential impact on treatment outcomes. The objectives of the study include investigating the specific microRNAs involved in regulating gene expression in cancer cells, elucidating the mechanisms by which they exert their effects, and exploring the therapeutic implications of targeting these microRNAs. The limitations of the study are discussed, including challenges related to experimental methodologies and data interpretation. The literature review section provides an in-depth analysis of existing research on microRNAs and their role in cancer, highlighting key findings and identifying gaps in knowledge. The research methodology outlines the experimental approaches used to study microRNA-mediated gene regulation in cancer cells, including cell culture techniques, molecular assays, and data analysis methods. The discussion of findings presents the results of the study, including the identification of specific microRNAs that regulate gene expression in cancer cells, the mechanisms by which they modulate gene expression, and the potential therapeutic targets that emerge from these findings. The implications of the study for cancer treatment are explored, with a focus on the development of novel therapeutic strategies that target microRNAs. In conclusion, this thesis provides valuable insights into the role of microRNAs in regulating gene expression in cancer cells and their potential as therapeutic targets. The study contributes to the growing body of knowledge on microRNA biology and cancer research, offering new perspectives on how microRNAs can be harnessed for improving cancer treatment outcomes.

Thesis Overview

The project titled "Investigating the role of microRNAs in regulating gene expression in cancer cells" aims to explore the intricate relationship between microRNAs and gene expression in cancer cells. MicroRNAs are small non-coding RNA molecules that play a crucial role in post-transcriptional gene regulation by binding to the messenger RNA (mRNA) of target genes, thereby influencing their expression levels. In cancer, dysregulation of microRNAs has been implicated in various aspects of tumorigenesis, including cell proliferation, apoptosis, metastasis, and drug resistance. This research project seeks to delve deeper into the specific mechanisms through which microRNAs modulate gene expression in cancer cells. By employing a combination of bioinformatics analysis, molecular biology techniques, and cell-based assays, the study aims to identify key microRNAs that are aberrantly expressed in different types of cancer and elucidate their downstream targets. Understanding the regulatory networks orchestrated by these microRNAs could provide valuable insights into the molecular pathways driving cancer progression and offer potential therapeutic targets for intervention. Furthermore, the project will investigate the functional consequences of manipulating the expression of specific microRNAs in cancer cells. By overexpressing or inhibiting selected microRNAs, the study will assess the impact on cell proliferation, migration, invasion, and response to anti-cancer therapies. This experimental approach will not only validate the regulatory role of microRNAs in cancer but also shed light on their potential as novel biomarkers or therapeutic agents for precision medicine strategies. Overall, this research overview underscores the significance of investigating the role of microRNAs in cancer biology. By unraveling the intricate interplay between microRNAs and gene expression in cancer cells, this project aims to contribute to our understanding of tumorigenesis mechanisms and potentially pave the way for the development of innovative diagnostic and therapeutic strategies in the fight against cancer.