Thesis Abstract

Abstract
MicroRNAs (miRNAs) are small non-coding RNAs that play pivotal roles in regulating gene expression, and their dysregulation has been implicated in various biological processes, including cancer progression. This thesis presents an in-depth analysis of the role of microRNAs in cancer progression, focusing on their molecular mechanisms, diagnostic potential, and therapeutic applications. Chapter one provides an introduction to the study by discussing the background of miRNAs, the problem statement, objectives, limitations, scope, significance, structure of the thesis, and definition of key terms. Chapter two presents a comprehensive literature review on ten key aspects related to miRNAs in cancer progression, including their involvement in tumor initiation, metastasis, and drug resistance. Chapter three outlines the research methodology, including the selection of miRNAs of interest, cell culture techniques, bioinformatics analysis, and experimental approaches used to investigate the functional roles of miRNAs in cancer cells. The chapter also details the methods employed for miRNA profiling and validation. Chapter four presents the findings from the study, discussing the differential expression of specific miRNAs in various cancer types, their target genes, and the functional consequences of miRNA dysregulation on cancer cell behavior. The chapter also explores the potential of miRNAs as diagnostic biomarkers and therapeutic targets in cancer treatment. Finally, chapter five offers a comprehensive conclusion and summary of the thesis, highlighting the key findings, implications for cancer research and treatment, and suggestions for future directions. Overall, this thesis provides a detailed analysis of the diverse roles of miRNAs in cancer progression and highlights their potential as promising targets for novel cancer therapies.

Thesis Overview

The project titled "The Role of MicroRNA in Cancer Progression: An In-Depth Analysis" aims to investigate the intricate role of MicroRNA (miRNA) in the progression of cancer. MicroRNAs are small non-coding RNA molecules that play crucial roles in regulating gene expression at the post-transcriptional level. Over the years, research has highlighted the significance of miRNAs in various biological processes, including tumorigenesis and cancer progression. The research will delve into the specific mechanisms by which miRNAs contribute to cancer progression, focusing on their involvement in key processes such as cell proliferation, apoptosis, metastasis, and drug resistance. By understanding how miRNAs interact with their target genes and signaling pathways, this study seeks to elucidate the complex network of molecular events that drive cancer development and progression. Through a comprehensive analysis of existing literature and experimental data, this project aims to identify specific miRNAs that are dysregulated in various types of cancer and evaluate their functional roles in promoting tumor growth and metastasis. By integrating bioinformatics tools and experimental validation techniques, the study will provide valuable insights into the diagnostic and therapeutic potential of targeting miRNAs in cancer treatment. Furthermore, the project will explore the emerging field of liquid biopsy and the use of circulating miRNAs as non-invasive biomarkers for cancer detection and monitoring. By investigating the expression profiles of circulating miRNAs in cancer patients, this research aims to evaluate their clinical utility in predicting treatment response and disease progression. Overall, this project on "The Role of MicroRNA in Cancer Progression: An In-Depth Analysis" seeks to advance our understanding of the intricate molecular mechanisms underlying cancer development and progression. By elucidating the role of miRNAs in driving tumorigenesis and metastasis, this study aims to contribute to the development of novel diagnostic and therapeutic strategies for improving cancer management and patient outcomes.