Thesis Abstract

Abstract
Cancer is a complex disease characterized by dysregulated gene expression, leading to uncontrolled cell growth and proliferation. MicroRNAs (miRNAs) have emerged as critical regulators of gene expression by modulating the translation and stability of target mRNAs. This thesis aims to explore the role of miRNAs in regulating gene expression in cancer cells, with a focus on understanding their involvement in cancer development and progression. Chapter 1 provides an introduction to the study, presenting the background of miRNAs, the problem statement regarding their role in cancer, the objectives of the study, limitations, scope, significance, structure of the thesis, and definitions of key terms. Chapter 2 presents a comprehensive literature review covering ten key aspects related to miRNAs and cancer, highlighting the current understanding of their mechanisms and significance in cancer biology. Chapter 3 outlines the research methodology, detailing the research design, sample collection and analysis methods, data interpretation techniques, and ethical considerations. This chapter also discusses the tools and techniques used to investigate the role of miRNAs in cancer gene expression regulation. Chapter 4 delves into the discussion of findings, presenting the results of the study, analyzing the impact of miRNAs on gene expression in cancer cells, and exploring the potential implications for cancer diagnosis and therapy. This chapter also addresses any challenges encountered during the research and suggests future directions for further investigation. Chapter 5 concludes the thesis by summarizing the key findings, discussing the implications of the research, and offering recommendations for future studies in this field. The thesis contributes to the growing body of knowledge on the role of miRNAs in cancer biology, providing insights into novel therapeutic targets and diagnostic strategies for cancer treatment. In conclusion, this thesis sheds light on the intricate regulatory mechanisms of miRNAs in cancer cells, highlighting their potential as diagnostic biomarkers and therapeutic targets in cancer therapy. The findings presented here contribute to a deeper understanding of the molecular pathways involved in cancer development and offer new avenues for personalized cancer treatment strategies.

Thesis Overview

The project titled "Exploring the role of microRNAs in regulating gene expression in cancer cells" aims to investigate the intricate involvement of microRNAs in the regulation of gene expression within cancer cells. MicroRNAs are small, non-coding RNA molecules that play crucial roles in post-transcriptional gene regulation by targeting messenger RNAs (mRNAs) for degradation or translational repression. In the context of cancer, dysregulation of microRNAs has been implicated in various aspects of tumorigenesis, including cell proliferation, apoptosis, metastasis, and drug resistance. This research endeavor seeks to delve into the specific mechanisms by which microRNAs influence gene expression in cancer cells and how these molecular interactions contribute to cancer development and progression. By examining the expression profiles of microRNAs in different types of cancer cells and correlating them with the expression levels of their target genes, this study aims to elucidate the regulatory networks underlying cancer pathogenesis. Furthermore, the project will explore the potential clinical implications of targeting microRNAs as a therapeutic strategy for cancer treatment. Understanding the role of microRNAs in cancer cell biology could lead to the development of novel diagnostic biomarkers and therapeutic interventions that specifically target the dysregulated gene expression patterns in cancer cells. Overall, this research project holds significant promise in advancing our understanding of the molecular mechanisms driving cancer progression and may pave the way for the development of more personalized and effective treatment approaches for cancer patients.