Thesis Abstract

Abstract
MicroRNAs (miRNAs) are small non-coding RNA molecules that play critical roles in post-transcriptional gene regulation, including in cancer cells. This thesis explores the intricate mechanisms by which miRNAs regulate gene expression in cancer cells, with a focus on understanding their role in tumorigenesis and potential implications for cancer therapy. Chapter 1 provides an introduction to the study, presenting the background of miRNAs, the problem statement, objectives, limitations, scope, significance, and the overall structure of the thesis. The definitions of key terms related to miRNAs and cancer biology are also outlined to facilitate understanding. Chapter 2 comprises a comprehensive literature review that delves into existing research on miRNAs and their involvement in cancer development and progression. Ten key themes are identified and discussed, shedding light on the current understanding of the field. Chapter 3 details the research methodology employed in this study. It covers aspects such as sample collection, experimental techniques, data analysis, and statistical methods. The chapter is divided into eight sections to provide a clear overview of the research approach. In Chapter 4, the findings of the study are extensively discussed. The results of experiments investigating the role of specific miRNAs in regulating gene expression in cancer cells are presented and analyzed. The implications of these findings for cancer biology and potential therapeutic strategies are also explored. Finally, Chapter 5 concludes the thesis by summarizing the key findings and insights gained from the study. The significance of the research in advancing our understanding of miRNA-mediated gene regulation in cancer cells is highlighted, along with recommendations for future research directions in this field. In conclusion, this thesis contributes to the growing body of knowledge on the role of miRNAs in cancer biology. By elucidating the mechanisms by which miRNAs regulate gene expression in cancer cells, this study offers valuable insights that may pave the way for the development of novel therapeutic approaches for cancer treatment.

Thesis Overview

The project titled "Exploring the role of microRNAs in regulating gene expression in cancer cells" focuses on investigating the intricate mechanisms by which microRNAs influence gene expression in cancer cells. MicroRNAs, small non-coding RNA molecules, have emerged as key regulators of gene expression by binding to target messenger RNAs and modulating their stability and translation. In cancer, dysregulation of microRNAs has been implicated in various aspects of tumorigenesis, including cell proliferation, migration, invasion, and resistance to therapy. The research will delve into the specific microRNAs involved in cancer pathogenesis and their target genes, elucidating how alterations in microRNA expression contribute to the development and progression of cancer. By dissecting the molecular interactions between microRNAs and their target genes, the study aims to uncover novel therapeutic targets for cancer treatment. Furthermore, the project will employ advanced molecular biology techniques, bioinformatics analysis, and cell culture experiments to investigate the functional roles of microRNAs in cancer cells. Through a comprehensive analysis of gene expression profiles and signaling pathways affected by microRNAs, the research seeks to provide a deeper understanding of the underlying molecular mechanisms driving cancer progression. Ultimately, this project aspires to contribute to the growing body of knowledge on the role of microRNAs in cancer biology and potentially pave the way for the development of targeted therapies that exploit the dysregulated microRNA networks in cancer cells. By shedding light on the complex interplay between microRNAs and gene expression in cancer, this research endeavor holds the promise of advancing our understanding of cancer biology and offering new avenues for personalized cancer therapy.