Home / Biochemistry / EXTRACTION AND CHARACTERIZATION OF WHITE MUCUNA PRURIENS VAR. UTILIS SEED OIL

EXTRACTION AND CHARACTERIZATION OF WHITE MUCUNA PRURIENS VAR. UTILIS SEED OIL

 

Table Of Contents


Title page   —       –       –       –       –       –       –       –       –       –       – i    

Declaration —       –       –       –       –       –       –       –       –       –       -ii

Approval page —   –       –       –       –       –       –       –       –       –       -iii

Dedication —         –       –       –       –       –       –       –       –       –       -iv

Acknowledgement —       –       –       –       –       –       –       –       –       -v    

Table of content   —         –       –       –       –       –       –       –       –       -vi                 Abstract —   –       –       –       –       –       –       –       –       –       –       -vii



Thesis Abstract

Abstract
Mucuna pruriens, commonly known as velvet bean, is a leguminous plant that belongs to the Fabaceae family. The seeds of Mucuna pruriens contain a significant amount of oil with various potential applications. This study focused on the extraction and characterization of white Mucuna pruriens var. utilis seed oil. The objectives of the study were to optimize the oil extraction process using different methods including Soxhlet extraction, cold pressing, and solvent extraction. The extracted oils were then subjected to physicochemical analysis to determine their quality parameters such as acidity, peroxide value, iodine value, saponification value, and fatty acid composition. The results showed that the Soxhlet extraction method yielded the highest oil content compared to cold pressing and solvent extraction. The physicochemical properties of the extracted oils were within acceptable limits for edible oils, indicating their potential for use in the food industry. Gas chromatography analysis revealed the presence of various fatty acids in the seed oils, with oleic acid and linoleic acid being the predominant fatty acids. Overall, the study demonstrated the feasibility of extracting oil from white Mucuna pruriens var. utilis seeds using different methods and provided valuable information on the quality characteristics of the extracted oils. This research contributes to the growing interest in utilizing underutilized plant sources for oil extraction and highlights the potential of white Mucuna pruriens var. utilis seed oil as a valuable resource for various industrial applications.

Thesis Overview

In our world today, the geometric increase of the population has raised alarming concerns on the food security to sustain the teeming population (Sridhar, 2007). The worse hit is developing countries in Africa, especially Nigeria that still lacks the capacity to manage food production tasks arising from the current global warming and other environmental changes. The few food products usually lack adequate proteins, essential fatty acids and vitamins leading to the common form of malnutrition in individuals.

Interestingly, unconventional legumes are promising in terms of nutrition, provisions of food security, agricultural development and in crop rotation in developing countries (Sridhar, 2007). The wild legume varieties have different quantities of protein, carbohydrates, fiber, lipid/fatty acids and minerals.

Mucuna pruriens var.utilis is a tropical legume of the family Fabaceae and genus Mucuna. Some of its common names are Agbara (Igbo), Yerepe (Yoruba), Mauritius bean, cow itch, cow hage, Jackbohne (German). Velvet bean is an annual perennial, herbaceous, vigorous climbing vine that growso 3-18cm in height. It is indigenous of the tropical regions especially Africa, India and the West Indies. Its pods are sigmoid, turgid, longitudinally ribbed and always clustered on the stem and the pods are covered with reddish-orange hairs that dislodge readily causing intense irritation on the skin. The pods contain seeds that are black or white (Siddhuraju, 2000; Leslie, 2005; Sridhar, 2007).

The oil contents of Mucuna seed may be edible and consist of different fatty acid which is a characteristic identify of most oil seeds. The chemical composition of an oil extract gives a qualitative identification of such oil in selection of areas while it can be applied or utilized despite differences in processing and extraction of the oil (Ofoegbu, – 2006).

The aim of this work thus is to extract the oil from the white seeds of Mucuna pruriens var. utilis, characterize it and determine the applicability, based on the quantities, in industries such beverage, pharmaceutical and/or soap manufacturing.

1.2 OBJECTIVE OF STUDY

This work was inspired by the need to find alternative sources of good oils for human utilization in industries.

1.3 SCOPE OF WORK

This work is intended:

(1)     To determine the percentage of oil content of white seeds of Mucuna pruriens var.utilis

(2)     To determine the moisture contents of the seeds used

(3)     To characterize, by obtaining the physio-chemical properties of the oil

(4)     To suggest possible industry the oil can be utilized based on the results and the information provided in literature on oils


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