Home / Biochemistry / PHYTOCHEMICAL ANALYSIS AND THE ANTI-INFLAMMATORY ACTIVITIES OF DICHLOROMETHANE FRACTION OF METHHANOL EXTRACT OF CRATEVA ADANSONII

PHYTOCHEMICAL ANALYSIS AND THE ANTI-INFLAMMATORY ACTIVITIES OF DICHLOROMETHANE FRACTION OF METHHANOL EXTRACT OF CRATEVA ADANSONII

 

Table Of Contents


Chapter ONE

1.1 Introduction
1.2 Background of study
1.3 Problem Statement
1.4 Objective of study
1.5 Limitation of study
1.6 Scope of study
1.7 Significance of study
1.8 Structure of the research
1.9 Definition of terms

Chapter TWO

2.1 Overview of Phytochemical Analysis
2.2 Importance of Phytochemicals in Medicine
2.3 Anti-inflammatory Activities of Plant Extracts
2.4 Crateva Adansonii: A Brief Overview
2.5 Previous Studies on Dichloromethane Fractions
2.6 Methodologies for Phytochemical Analysis
2.7 Techniques for Anti-inflammatory Activity Assessment
2.8 Role of Solvents in Extracting Phytochemicals
2.9 Factors Affecting Phytochemical Composition
2.10 Mechanisms of Anti-inflammatory Actions

Chapter THREE

3.1 Research Methodology Overview
3.2 Selection of Research Design
3.3 Sampling Techniques
3.4 Data Collection Methods
3.5 Phytochemical Analysis Procedures
3.6 Anti-inflammatory Activity Assays
3.7 Statistical Analysis Techniques
3.8 Ethical Considerations in Research

Chapter FOUR

4.1 Analysis of Phytochemical Composition
4.2 Evaluation of Anti-inflammatory Properties
4.3 Comparison with Other Plant Extracts
4.4 Correlation between Phytochemicals and Activities
4.5 Impact of Extraction Methods on Results
4.6 Discussion on Variability in Phytochemical Content
4.7 Interpretation of Statistical Findings
4.8 Implications for Future Research

Chapter FIVE

5.1 Summary of Findings
5.2 Conclusion and Recommendations
5.3 Contributions to Knowledge
5.4 Practical Applications of the Study
5.5 Areas for Further Research

Thesis Abstract

Inflammation is a complex biological response of vascular tissue to harmful stimuli such as pathogen, damage cells or irritants (Ryan & Majno, 1983). The urgency generated by increased rate of stroke, atherosclerosis attribute due to prolonged use of cyclooxygenase-1 and Cyclooxygenase- 2 inhibitors have accelerated anti-inflammatory drug research over the last decade while synthetic pharmaceutical agents continued to dominate research (Ryan & Majno, 1983). Attention increasingly has been directed to natural products. These are often more affordable and available and sometimes are perceived as more effective than conventional anti- inflammatory drugs.Anti-inflammation was carried out using 12 rats which was divided into three groups of 4 rats each. Group 1 and 2 served as the negative and positive control respectively. Group 3 received 25mg/kg b.w. of the dichloromethane fraction of methanol extract of Crateva adansonii showed significance anti-inflammatory activity when compared with the standard difference used.



Thesis Overview

Inflammation is one of body’s natural ways of protecting itself. It is also an essential reaction of the body to infection. Too little of an inflammatory response, and the body is unable to repel microbial invasions or heal injuries. Too much of an inflammatory response, and the immune system begins attacking the body’s own organs eventually leading to chronic disease (Sears, 2000). Anti-Inflammatory refers to the property of a substance or treatment that reduces inflammation. There are obvious clinical markers of inflammation which include pain, redness, fever, swelling and loss of function (Pai et al. 2004). However if inflammation is allowed to continue unchecked it may results in neurodegenerative disease or cancer (Pai et al. 2004). A variety of safe and effective anti-inflammatory agents are available including non-steroidal anti-inflammatory drugs, corticosteroids.



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