Thesis Abstract

Abstract
Cancer is a complex and multifaceted disease that continues to pose a significant challenge to healthcare systems worldwide. In recent years, microRNAs (miRNAs) have emerged as key regulators of gene expression, playing critical roles in various biological processes, including cancer development and progression. This thesis aims to investigate the role of miRNAs in regulating gene expression in cancer cells, with a focus on understanding their mechanisms of action and potential implications for cancer therapy. The introduction provides a comprehensive overview of cancer biology, highlighting the importance of gene regulation in cancer development. The background of the study delves into the current understanding of miRNAs and their role in post-transcriptional gene regulation. The problem statement identifies the gaps in knowledge regarding the specific mechanisms by which miRNAs regulate gene expression in cancer cells. The objectives of the study are to elucidate the functional significance of specific miRNAs in cancer cells, characterize their target genes, and investigate the impact of miRNA dysregulation on cancer progression. The limitations of the study are acknowledged, including the challenges associated with experimental validation of miRNA-target interactions and the complexity of cancer heterogeneity. The scope of the study encompasses in vitro and in vivo experiments using cancer cell lines and animal models to investigate the functional roles of miRNAs in cancer biology. The significance of the study lies in the potential for identifying novel therapeutic targets and biomarkers for cancer diagnosis and prognosis. The structure of the thesis outlines the organization of the subsequent chapters, including a literature review that synthesizes existing knowledge on miRNAs and cancer, a research methodology detailing the experimental approaches employed, a discussion of the findings, and a conclusion summarizing the key insights and implications of the study. Overall, this thesis aims to advance our understanding of the intricate interplay between miRNAs and gene expression in cancer cells, with the ultimate goal of contributing to the development of innovative therapeutic strategies for cancer treatment.

Thesis Overview