1. Introduction
  1.1 Background and significance
  1.2 Objectives of the study
2. Protein Engineering Techniques
  2.1 Rational design approaches
  2.2 Directed evolution methods
  2.3 Combinatorial protein libraries
3. Modification of Protein Structure
  3.1 Site-directed mutagenesis
  3.2 Protein domain swapping
  3.3 Protein fusion and chimeric proteins
4. Engineering Protein Function
  4.1 Enzyme engineering and catalytic activity
  4.2 Protein-protein interaction engineering
  4.3 Protein-based biosensors and diagnostics
5. Enhancing Protein Stability
  5.1 Protein folding and stability determinants
  5.2 Protein stabilization strategies
  5.3 Engineering proteins for extreme conditions