Home / Human Nutrition and Dietetics / Monostaining of kidney and liver tissues with extracts of tumeric (curcuma longa) and zobo (hibiscus sabdariffa)

Monostaining of kidney and liver tissues with extracts of tumeric (curcuma longa) and zobo (hibiscus sabdariffa)

 

Table Of Contents


Chapter ONE

1.1 Introduction
1.2 Background of Study
1.3 Problem Statement
1.4 Objective of Study
1.5 Limitation of Study
1.6 Scope of Study
1.7 Significance of Study
1.8 Structure of the Research
1.9 Definition of Terms

Chapter TWO

2.1 Overview of Monostaining
2.2 Historical Perspectives on Tumeric and Zobo
2.3 Chemical Composition of Tumeric and Zobo
2.4 Health Benefits of Tumeric and Zobo
2.5 Previous Studies on Monostaining Techniques
2.6 Monostaining Procedures
2.7 Comparison of Monostaining Methods
2.8 Applications of Monostaining in Research
2.9 Challenges in Monostaining Techniques
2.10 Future Directions in Monostaining Research

Chapter THREE

3.1 Research Methodology Overview
3.2 Selection of Kidney and Liver Tissues
3.3 Preparation of Tumeric and Zobo Extracts
3.4 Monostaining Procedure for Tissues
3.5 Experimental Design and Controls
3.6 Data Collection Methods
3.7 Statistical Analysis Techniques
3.8 Ethical Considerations in Research

Chapter FOUR

4.1 Overview of Research Findings
4.2 Analysis of Monostaining Results
4.3 Comparison of Tumeric and Zobo Staining
4.4 Cellular Effects of Monostaining
4.5 Interpretation of Tissue Reactions
4.6 Discussion on Variability in Staining
4.7 Implications of Findings in Medical Field
4.8 Recommendations for Further Studies

Chapter FIVE

5.1 Conclusion and Summary
5.2 Summary of Research Objectives
5.3 Key Findings Recap
5.4 Contributions to Scientific Knowledge
5.5 Practical Applications of Research
5.6 Limitations and Future Research Directions

Project Abstract

Monstaining is a special kind of staining procedure that highlights single cells. Most stains used in histopathology are synthetic, toxic and costly for use hence this study was carried out at the histopathology laboratory of Imo state university to research more on the probability of replacing routine toxic and costly stains for natural, non toxic, less costly and effective stains that can highlight observably single cellular structures when viewed microscopically. Twelve sections were used for this study (6 liver and 6 kidney sections), obtained from the federal medical center Owerri of which was divided into four groups of liver and kidney sections respectively. This division was according to stains to be used on both sections either with haematoxylin and eosin or prepared adjusted PH extracts of zobo and turmeric viz The group 1 (3 liver sections) were stained with haematoxylin and eosin, group 2 (3liver sections) stained with zobo and turmeric plant extracts, group 3 (3kidney sections) stained with haematoxylin and eosin stains and group 4 (3kidney sections) stained with zobo and turmeric plant extracts. After analysis it was concluded that zobo and turmeric were not very good stains in highlighting single cell structures when compared to routinely used haematoxylin and eosin stains.

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