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Comparative study on the antimicrobial properties of chilli pepper (capsicum annuum) on staphylococcus aureus and escherichia coli

 

Table Of Contents


Chapter ONE

1.1 Introduction
1.2 Background of Study
1.3 Problem Statement
1.4 Objective of Study
1.5 Limitation of Study
1.6 Scope of Study
1.7 Significance of Study
1.8 Structure of the Research
1.9 Definition of Terms

Chapter TWO

2.1 Overview of Antimicrobial Properties
2.2 Chilli Pepper (Capsicum Annuum) as a Natural Antimicrobial Agent
2.3 Staphylococcus Aureus: Characteristics and Risks
2.4 Escherichia Coli: Understanding the Bacterial Strain
2.5 Previous Studies on Chilli Pepper's Antimicrobial Activity
2.6 Mechanisms of Action of Chilli Pepper Compounds on Bacteria
2.7 Factors Affecting Antimicrobial Efficacy of Chilli Pepper
2.8 Comparative Studies on Staphylococcus Aureus and Escherichia Coli
2.9 Importance of Studying Antimicrobial Properties for Public Health
2.10 Future Research Directions in Natural Antimicrobial Agents

Chapter THREE

3.1 Research Methodology Overview
3.2 Research Design and Approach
3.3 Sampling Methods and Criteria
3.4 Data Collection Techniques
3.5 Experimental Setup and Variables
3.6 Data Analysis Procedures
3.7 Ethical Considerations in Research
3.8 Validation and Reliability Measures

Chapter FOUR

4.1 Data Analysis and Interpretation
4.2 Antimicrobial Effects of Chilli Pepper on Staphylococcus Aureus
4.3 Antimicrobial Effects of Chilli Pepper on Escherichia Coli
4.4 Comparison of Antimicrobial Activity between the Two Bacterial Strains
4.5 Discussion on Factors Influencing Antimicrobial Efficacy
4.6 Implications of Findings in Public Health
4.7 Recommendations for Further Research
4.8 Comparison with Previous Studies

Chapter FIVE

5.1 Conclusion and Summary
5.2 Recap of Research Objectives and Findings
5.3 Contributions to Knowledge and Practice
5.4 Limitations and Areas for Improvement
5.5 Practical Applications and Recommendations
5.6 Significance of the Study in the Field
5.7 Concluding Remarks and Final Thoughts

Project Abstract

Antimicrobial activities of fruit extract from Capsicum annuum (chilli pepper) were examined against two organisms namely Staphylococcus aureus and Escherichia coli. These organisms are gram-positive and gram-negative bacteria respectively. The antimicrobial property was investigated using disc diffusion method (Sensitive test). The result of the extract prepared with cold water, hot water and ethanol with different concentrations of 50ug/ml, 25ug/ml, 12.5ug/ml, 6.25ug/ml and 3.125ug/ml were obtained as well as the minimum inhibition of 12.5mm, 6.25mm and 6.25mm respectively against Staphylococcus aureus while Escherichia coli has maximum inhibition concentration of 25mm, 12.5mm and 6.25mm using the same concentration as above. Both organisms were inhibited by the extract from chilli pepper.

Project Overview

1.0 INTRODUCTION

       Capsicum annuum (chilli pepper) is used as spice in cookery. They are sometimes dried, ground and used for their strong flavour and preservative effect. The two pungent compounds found in Capsicum species which are capsaicin and dihydrocapsaicin have antimicrobial effects.

      Fresh chilli peppers are very rich in vitamin C: 94mg which makes them very effective as immune system stimulants and healing agents especially for cellular damage. Many fork remedies recommended chilli pepper in wound cleaning preparations for gangrene and open sores and even as styptic, though more modern sources generally advise against using chilli on broken skin. Chillies diminish most of their vitamin C while drying thereby increasing the vitamin A content by 100 times. Vitamin A is a powerful anti-oxidant and anti-inflammatory agent. (Anguilar et al., 2010).

      Surprisingly, it has been shown that chillies do not aggravate or cause stomach ulcers. In fact they seem to have a preventative effect, as stomach ulcers are mostly caused by bacteria hence chilli’s antimicrobial actions kill such bacteria (Rogers, 2008).

      Chilli peppers can be very pungent and irritating. The degree of pungency can be adjusted by removing the seeds and white skin (placenta) which is where most of the capsaicin is concentrated.

      Chilli peppers are used for other health purposes like headaches, fever, colds, nasal catarrh, high blood pressure, stomach and digestive problems, sore throat etc.

      Extraction of the antimicrobial and antioxidant content of chilli pepper can be done by solvent extraction method being the simplest method. This method involves grinding the dried chilli pepper and mixing with an appropriate solvent which dissolves out the essential content of the pepper. Examples of such solvents include: ethanol, methanol, acetic acid and acetone (Duran et al., 2009).

1.1 Statement of Problem

Bacteria, Staphylococcus aureus and Escherichia coli have developed resistance to some antibacterial drug. This lead to the study on the antimicrobial activity of Chilli pepper to know whether it can be used as an alternative therapy for is faction caused by these organisms.

1.2     Objectives

–     To identify the antimicrobial properties of Capsicum annuum.

–     To determine the comparative study of Capsicum annuum.

1.3 Significance of the Study

      This work will help to unveil the potential use, application and extract from chili pepper to fight against spoilage and pathogenic microorganisms in food and body systems respectively.

1.4 Scope of Study

This project is based on antimicrobial activity of Staphylococcus aureus and Escherichia coli isolated from clinical sample (wound swab) collected from University of Nigeria Teaching Hospital Ituku Ozalla.  

1.5 Limitations of the Study

      This work is limited to the study on the antimicrobial properties of chili pepper. There were some difficulties in carrying out this research work which includes financial constrain, unavailability of laboratory equipment and time factor.



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