EXTRACTION AND ESTIMATION OF VITAMIN C CONTENT IN TANGERINE JUICE
Table Of Contents
Cover page
Title page
Certification
Dedication
Acknowledgement
Abstract
Organization of the work
Table of Contents
Chapter ONE
Introduction
Scientific classification of tangerine
History and origin of tangerine
Vitamin C in food and fruits
Food and fruits that provides vitamin c
Scope of study
Chapter TWO
Literature Review
Plant description
General culture (cultivation) of tangerine
Soil and climatic requirements
Preparing the mound
Planting of tangerines seed
Looking after the trees
i. Pruning
ii. Weed elimination
iii. Maturity
iv. Harvesting
v. Marketing
vi. Uses of Tangerine fruits
vii. Four main horticultural groups (varieties) of tangerine
Nutritional value of tangerine
Vitamin C
Factors affecting the vitamin C content of citrus fruits
Forms of vitamin C found in Dietary (food) supplements
Functions of vitamin C
Functions of other vitamins
Role of vitamins in metabolism
Deficiency symptoms of vitamin C
Chapter THREE
Materials and methods
Materials/Equipments
Chemicals reagents
Collection of sample
Sterilization of materials
Extraction of juice from tangerine fruits
Ph of extract
Experimental methods
Experimental procedures
Chapter FOUR
Experimental results
Chapter FIVE
Discussion
Conclusion
References
Project Abstract
The estimation of vitamin C
in Tangerine Juice was investigated, the results obtained using
titrimetric method shows that one tangerine fruit juice contains
approximately 10mg of vitamin C content, and the 19 fruits sample used
in the experiment contains about 190mg of vitamin c, from other survey
in literature, tangerine juice was discovered to contain other nutrient
such as Vitamin B2, B12, Vit. E, Folate, Folic
Acid in little quantities but contains high quantity of Vitamin C, which
shows that tangerine fruit juice is a good source of vitamin C and it
is advised to take about 6 fruits of tangerine per day to meet the RDA
standard required.
Project Overview
INTRODUCTION
SCIENTIFIC C
INTRODUCTION
SCIENTIFIC CLASSIFICATION OF TANGERINE
Kingdom: Plantae
Division: Magnoliophyta
Class: Magnoliopsida
Order: Sapindales
Family: Rutaceae
Genus: Citrus
Species: C reticulate
Binomial name: Citrus reticulate
1.1 HISTORY AND ORIGIN OF TANGERINE
Tangerine,
which are native to Japan since the sixteenth century; belong to the
mandarine orange family that has flourished in the orient for centuries.
The tangerine is named from tangers, the North African part from which
this citrus was originally shipped to Florida. Tangerine is most
abundantly grown in Japan, southern China, Indian and the East Indies,
and is esteemed for home consumption in Australia and the United States.
Tangerines gravitated to the Western world by small steps taken by
individuals interested in certain spread, can be roughly traced in the
chronology of separate introductions., two varieties from canton were
taken to cultivation in the Mediterranean area by 1850, were all
established in Italy.
Sometimes between 1840 and 1850, the willow leaf or china Mandarin was
imported by the Italian Consular and planted at the consulate in New
Orleans. It was carried from there to Florida and later reached
California. The -Owari†Satsuma arrived from Japan, first in 1876 and
next in 1878 and nearly a million buddle trees from 1908 to 1911 for
planting in Gulf States. Six fruits of the -King Mandarin were sent,
from Saigon in 1882 to a Dr. Magee at the River State, in California.
They latter sent two (2) seedlings to winter park, Florida. Seeds of the
-Oneco†Mandarin were obtained from India by the nurseryman, P. W.
Reasoner in 1882. in 1892 (or 1893) two (2) fruits of Ponkan were sent
from China to J. C. Barrington of MC Meskin, Florida and seedlings from
there were distributed and led to commercial propagation.
The distribution of the Mandarin Orange family seedling from Florida
led to the introduction of tangerine (Mandarin family) into African
through the white men who engage in African human slavery trade and also
through some white men who colonizes the African Countries. Because of
the edible and portability nature of tangerine, the white men find it
very easily to be transported and these distributions also lead to the
introduction of tangerine into the West African and then to Nigeria.
Today in Nigeria tangerine trees are found in so many orchards and other
places in the community being grounded or planted near peoples home or
surroundings, because it mostly grown through explosive mechanism. Today
in Nigeria, ripe tangerines fruits are purchased directly form their
trees or from local or villages market were they are sold and it is
mostly meant for the consumption purpose because of its edible in
nature.
1.2 VITAMIN C IN FOODS AND FRUITS
The
content of vitamin C is widely distributed in fruits and vegetables. A
portion is lost after prolonged storage of unprocessed fruits and
vegetables, but it is partially preserved (half or greater) by most
means of food processing which includes boiling, steaming, pressure
cooking, preserving jams, and jellies, freezing, dehydration and
canning. Utilization of the vitamin C is increased during pregnancy and
lactation and in thyrotoxicosis and absorption is decreased in diarrhea
states and in achlohydria.
Vitamin C is highly sensitive to air, water and temperature. About 25%
of the vitamin C in vegetable can be lost simply by blanching (boiling
or steaming the food for a few minutes). This same degree of loss occurs
in the freezing and unthawing of vegetables and fruits. Cooking of
vegetables and fruits of longer periods of time (10-20 minutes) can
result in a loss of over one half the total vitamin C content. When
fruits and vegetables are canned and then reheated only 1/3 of the
original vitamin C content may be left. In general, an unripe food is
much lower in vitamin C than a ripe one, but provided that the food is
matured and ripe, the vitamin C content is high when the food is younger
at the time of harvest. Vitamin C decreases during the ripening process
immature fruits have the highest levels of vitamin c.
1.3 FOODS AND FRUITS THAT PROVIDE VITAMIN C
Excellent
foods and fruits with high level of vitamin C includes broccoli, bell
peppers, kale Cauli flower, strawberries, lemon, mustard and turnip
greens, Brussels sprouts, papaya, chard, cabbage, spinach, kiwifruit,
snow peas, cantaloupe, oranges, grape fruits, lime, tomatoes,
tangerines, Zucchini, raspberries, a asparagus, celery, pineapples,
lettuce, water melon, fennel, peppermint, parsley and other ripe
fruits.
1.4 SCOPE OF STUDY
Numerous
analytical techniques have been reported in the literature for the
determination of vitamin C in different citrus juice. These includes
Titrimetric, Fluorometric, Complexometric methods, liquid
Curomatography, high-performance liquid chromatography,
Spectrophotometric, Amperometric and enzymatic. Most of these methods
overestimate the levels of vitamin C in different citrus juice due to
the presence of oxidizable species other than vitamin C. the vitamin C
levels in some tropical food samples have been reported by several
investigators. The methods commonly employed in the determination and
estimation of vitamin C in citrus juice was titrimetric or
Spectrophotometric method. Although titrimetric methods are simple to
use in the determination of vitamin C, difficulties are encountered with
commonly used titrants and interferences often occur with coloured
samples.
Therefore, the estimation of vitamin C in tangerine juice will be
assessed to determine the quantity of vitamin C in tangerine juice. This
will be done through titrimetric analysis.
CLASSIFICATION OF TANGERINE
Kingdom: Plantae
Division: Magnoliophyta
Class: Magnoliopsida
Order: Sapindales
Family: Rutaceae
Genus: Citrus
Species: C reticulate
Binomial name: Citrus reticulate
1.1 HISTORY AND ORIGIN OF TANGERINE
Tangerine,
which are native to Japan since the sixteenth century; belong to the
mandarine orange family that has flourished in the orient for centuries.
The tangerine is named from tangers, the North African part from which
this citrus was originally shipped to Florida. Tangerine is most
abundantly grown in Japan, southern China, Indian and the East Indies,
and is esteemed for home consumption in Australia and the United States.
Tangerines gravitated to the Western world by small steps taken by
individuals interested in certain spread, can be roughly traced in the
chronology of separate introductions., two varieties from canton were
taken to cultivation in the Mediterranean area by 1850, were all
established in Italy.
Sometimes between 1840 and 1850, the willow leaf or china Mandarin was
imported by the Italian Consular and planted at the consulate in New
Orleans. It was carried from there to Florida and later reached
California. The -Owari†Satsuma arrived from Japan, first in 1876 and
next in 1878 and nearly a million buddle trees from 1908 to 1911 for
planting in Gulf States. Six fruits of the -King Mandarin were sent,
from Saigon in 1882 to a Dr. Magee at the River State, in California.
They latter sent two (2) seedlings to winter park, Florida. Seeds of the
-Oneco†Mandarin were obtained from India by the nurseryman, P. W.
Reasoner in 1882. in 1892 (or 1893) two (2) fruits of Ponkan were sent
from China to J. C. Barrington of MC Meskin, Florida and seedlings from
there were distributed and led to commercial propagation.
The distribution of the Mandarin Orange family seedling from Florida
led to the introduction of tangerine (Mandarin family) into African
through the white men who engage in African human slavery trade and also
through some white men who colonizes the African Countries. Because of
the edible and portability nature of tangerine, the white men find it
very easily to be transported and these distributions also lead to the
introduction of tangerine into the West African and then to Nigeria.
Today in Nigeria tangerine trees are found in so many orchards and other
places in the community being grounded or planted near peoples home or
surroundings, because it mostly grown through explosive mechanism. Today
in Nigeria, ripe tangerines fruits are purchased directly form their
trees or from local or villages market were they are sold and it is
mostly meant for the consumption purpose because of its edible in
nature.
1.2 VITAMIN C IN FOODS AND FRUITS
The
content of vitamin C is widely distributed in fruits and vegetables. A
portion is lost after prolonged storage of unprocessed fruits and
vegetables, but it is partially preserved (half or greater) by most
means of food processing which includes boiling, steaming, pressure
cooking, preserving jams, and jellies, freezing, dehydration and
canning. Utilization of the vitamin C is increased during pregnancy and
lactation and in thyrotoxicosis and absorption is decreased in diarrhea
states and in achlohydria.
Vitamin C is highly sensitive to air, water and temperature. About 25%
of the vitamin C in vegetable can be lost simply by blanching (boiling
or steaming the food for a few minutes). This same degree of loss occurs
in the freezing and unthawing of vegetables and fruits. Cooking of
vegetables and fruits of longer periods of time (10-20 minutes) can
result in a loss of over one half the total vitamin C content. When
fruits and vegetables are canned and then reheated only 1/3 of the
original vitamin C content may be left. In general, an unripe food is
much lower in vitamin C than a ripe one, but provided that the food is
matured and ripe, the vitamin C content is high when the food is younger
at the time of harvest. Vitamin C decreases during the ripening process
immature fruits have the highest levels of vitamin c.
1.3 FOODS AND FRUITS THAT PROVIDE VITAMIN C
Excellent
foods and fruits with high level of vitamin C includes broccoli, bell
peppers, kale Cauli flower, strawberries, lemon, mustard and turnip
greens, Brussels sprouts, papaya, chard, cabbage, spinach, kiwifruit,
snow peas, cantaloupe, oranges, grape fruits, lime, tomatoes,
tangerines, Zucchini, raspberries, a asparagus, celery, pineapples,
lettuce, water melon, fennel, peppermint, parsley and other ripe
fruits.
1.4 SCOPE OF STUDY
Numerous
analytical techniques have been reported in the literature for the
determination of vitamin C in different citrus juice. These includes
Titrimetric, Fluorometric, Complexometric methods, liquid
Curomatography, high-performance liquid chromatography,
Spectrophotometric, Amperometric and enzymatic. Most of these methods
overestimate the levels of vitamin C in different citrus juice due to
the presence of oxidizable species other than vitamin C. the vitamin C
levels in some tropical food samples have been reported by several
investigators. The methods commonly employed in the determination and
estimation of vitamin C in citrus juice was titrimetric or
Spectrophotometric method. Although titrimetric methods are simple to
use in the determination of vitamin C, difficulties are encountered with
commonly used titrants and interferences often occur with coloured
samples.
Therefore, the estimation of vitamin C in tangerine juice will be
assessed to determine the quantity of vitamin C in tangerine juice. This
will be done through titrimetric analysis.