Chapter ONE

1.1 Introduction
1.2 Background of Study
1.3 Problem Statement
1.4 Objective of Study
1.5 Limitation of Study
1.6 Scope of Study
1.7 Significance of Study
1.8 Structure of the Research
1.9 Definition of Terms

Chapter TWO

2.1 Overview of In Vitro Regeneration
2.2 History of Treculia Africana Decne
2.3 Importance of Plant Regeneration
2.4 Factors Affecting Regeneration Process
2.5 Types of Plant Tissue Culture Media
2.6 Murashige and Skoog (MS) Medium
2.7 Gamborg et al. (B5) Medium
2.8 Previous Studies on Treculia Africana Decne. Regeneration
2.9 Challenges in Regeneration Techniques
2.10 Future Prospects in Plant Regeneration Research

Chapter THREE

3.1 Research Methodology Overview
3.2 Selection of Embryo Explants
3.3 Preparation of Murashige and Skoog Medium
3.4 Preparation of Gamborg et al. Medium
3.5 Culture Conditions and Growth Parameters
3.6 Inducing Regeneration in Tissue Cultures
3.7 Data Collection and Analysis Methods
3.8 Statistical Tools for Data Interpretation

Chapter FOUR

4.1 Analysis of Regeneration Efficiency
4.2 Comparison of MS and B5 Media Results
4.3 Morphological and Physiological Changes in Regenerated Plants
4.4 Genetic Stability of Regenerated Plants
4.5 Factors Influencing Regeneration Success
4.6 Discussion on Regeneration Protocols
4.7 Implications of Findings in Plant Biotechnology
4.8 Future Research Directions

Chapter FIVE

5.1 Conclusion and Summary
5.2 Recap of Research Objectives
5.3 Key Findings and Contributions
5.4 Practical Applications of the Study
5.5 Recommendations for Further Research