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</p><p>1.0 Introduction</p><p>1.1 Aim of Study</p><p>1.2 Literature Review</p><p>1.3 Phytochemicals</p><p>1.3.1 Alkaloids</p><p>1.3.2 Flavonoids</p><p>1.3.2.1 Antioxidant activities</p><p>1.3.2.2 Antimicrobial activities</p><p>1.3.2.3 Antibacterial activities</p><p>1.3.2.3 Antiatherosclerotic effects</p><p>1.3.3 Saponins</p><p>1.3.4 Tannins</p><p>
Chapter TWO
</p><p>2.0 Materials and Methods</p><p>2.1 Materials Used</p><p>2.2 Collection and Identification of samples</p><p>2.3 Sample preparation</p><p>2.3.1 Drying of the leaves</p><p>2.3.2 Pulverisation of the leaves</p><p>2.3.3 Extraction Procedure</p><p>2.3.4 Filtration Process</p><p>2.3.5 Concentration Process</p><p>2.4 Phytochemical Analysis</p><p>2.4.1 Oualitative Analysis</p><p>2.4.1.1 Test for Alkaloids</p><p>2.4.1.2 Test for Phytosterol</p><p>2.4.1.3 Test for Flavonoids</p><p>2.4.1.4 Test for Saponin</p><p>2.4.1.5 Test for Phenol and Tannin</p><p>2.4.2 Ouantitative Analysis</p><p>2.4.2.1 Determination of flavonoid content</p><p>2.4.2.2 Determination of tannins content</p><p>2.4.2.3 Determination of Saponins content</p><p>2.4.2.4 Determination of flavonoid content</p><p>2.4.2.5 Determination of Phenolic content</p><p>2.4.2.6 Determination of Reducing Power</p><p>
Chapter THREE
</p><p>3.0 Results</p><p>
Chapter FOUR
</p><p>4.0 Discussion and Conclusion</p><p>4.1 Discussion</p><p>4.2 Conclusion</p><p>References</p><p>LIST OF TABLES</p><p>Table 1 – Showing the qualitative analysis results of phytochemical constituents of <em>Ocimum grattissimum </em></p><p>Table 2 – Showing the quantitative analysis of phytochemical constituents of <em>Ocimum grattissimum </em></p><p>Table 3 – Showing the flavonoids determination</p><p>Table 4 – Showing the tannin determination</p><p>Table 5 – Showing the total phenol determination</p><p>Table 6 – Showing the ascorbic acid absorbance</p><p>Table 7 – Bioactivity of compounds detected through GC-MS screening of methanolic extract of scent leaves</p><p>LIST OF FIGURES</p><p>Figxure 1 – Graph showing the flavonoids determination</p><p>Figure 2 – Graph showing the tannin determination</p><p>Figure 3 – Graph showing the total Phenol determination</p><p>Figure 4 – Graph showing the reducing standard curve.</p>
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