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Isolation, identification and antibiogram of staphylococcus aureus isolated from cow meat

 

Table Of Contents


Chapter 1

1.1 Introduction
1.2 Background of study
1.3 Problem Statement
1.4 Objective of study
1.5 Limitation of study
1.6 Scope of study
1.7 Significance of study
1.8 Structure of the research
1.9 Definition of terms

Chapter 2

2.1 Overview of Staphylococcus aureus
2.2 History of Staphylococcus aureus research
2.3 Pathogenicity of Staphylococcus aureus
2.4 Transmission of Staphylococcus aureus
2.5 Antibiotic resistance in Staphylococcus aureus
2.6 Methods for isolation of Staphylococcus aureus
2.7 Identification techniques of Staphylococcus aureus
2.8 Role of Staphylococcus aureus in foodborne illnesses
2.9 Antimicrobial susceptibility testing of Staphylococcus aureus
2.10 Current research trends in Staphylococcus aureus

Chapter 3

3.1 Research design and rationale
3.2 Sampling methods
3.3 Data collection procedures
3.4 Data analysis techniques
3.5 Quality control measures
3.6 Ethical considerations
3.7 Research limitations
3.8 Research validity and reliability

Chapter 4

4.1 Overview of research findings
4.2 Isolation of Staphylococcus aureus from cow meat
4.3 Identification of Staphylococcus aureus isolates
4.4 Antibiogram analysis of Staphylococcus aureus
4.5 Comparison with existing literature
4.6 Discussion on antibiotic resistance patterns
4.7 Implications of findings on public health
4.8 Recommendations for future research

Chapter 5

5.1 Summary of research findings
5.2 Conclusion of the study
5.3 Contributions to the field
5.4 Practical implications
5.5 Recommendations for stakeholders

Project Abstract

The study was carried out with aim to isolate Staphylococcus aureus from cow meat and determine the antibiogram pattern of S. aureus. Three samples of cow meat from three different locations (Gwagwalada market, Abattoir market and Kasuan Dare) in Gwagwalada were collected to isolate S. aureus from the samples. The organisms isolated were Salmonella spp, Escherichia coli, Staphylococcus aureus and Listeria monocytogenes. Several biochemical tests were carried out to identify, the S. aureus isolates like Catalse test (positive), Manitol test (positive), Coagulase test (positive) and Oxidase test (negative). The antibiogram pattern of S. aureus was evaluated using commercially prepared disk. The antibiotics tested were Pefloxacin (Pef), Streptomycin (S), Ampiclox (Apx), Amoxacilin (Am) and Ciprofloxacin. The pattern indicated that the overall S. aureus were resistant to Pef, S, Cpx and less resistant to Am and Apx. Results clearly suggested a possibility of potential public health threat of S. aureus resulting from contamination of cow meat with pathogenic bacteria which is mainly due to unhygienic processing, handling, and unhygienic environment.

Project Overview

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