Pages
Title Page --------ii
Abstract--------iii
Acknowledgement-------iv
Certification--------v
Dedication--------vi
Table of contents-------vii
List of Tables--------ix
List of Figures--------x
INTRODUCTION ------1
1.1 Objectives of the Study-----5
LITERATURE REVIEW ------6
2.1 Potential of Guinea grass (Panicum Maximum) as forage in livestock production ---6
2.2 Cultivars of Guinea grass----6
2.3 Nutritional qualities and chemical composition of Guineagrass-------7
2.4 Effect of stage of growth and frequency of cutting on the yield and chemical composition of Guinea grass-10
2.5 Silage as an alternative source of roughage--12
2.6 Silage fermentation and preservation--13
2.6.1 Fermentation process -----13
2.6.2 Six Steps during ensiling, storage and feed-out of fermented forages-----15
2.6.3 Additions to silage-----18
2.6.4 Addition of water to increase moisture content -19
2.6.5 Inhibition of bacteria and mold growth --19
2.6.6 Culturing" silage (inoculants)---20
2.6.7 Qualities of a good silage----20
2.7 Grasses ensiled with fresh sugarcane crush--21
2.8 History and methodological considerations on in vitro gas production techniques---23
2.8.1 Effect of sample size on in vitro gas production-24
2.8.2 Effect of agitation of the medium on in vitro gas Production------25
2.8.3 Effect of changes in atmospheric pressure and of venting gas during incubation on in vitro gas production-26
2.9 Determination of nutritive value of ruminant feeds using in vitro gas production technique---27
2.10 Alternative feed resources by Gas production and rumen Fermentation in vitro-----28
2.11 In vitro digestion, isolation and identification of rumen bacteria in silage using fermentation technique---31
Chapter THREE
3.0 MATERIALS AND METHODS---37
3.1 Preparation of grass for silage----37
3.2 Determination of silage quality---38
3.3 Silage acceptability study----38
3.4 Chemical analysis-----39
3.4.1 Moisture content determination ---39
3.4.2 Ash determination-----39
3.4.3 Crude protein determination----40
3.4.4 Neutral Detergent Fibre and Acid Detergent Fibre Determination------41
3.4.4.1 Determination of cell wall fraction (NDF and ADF)-41
3.4.4.2 Composition of the Neutral Detergent Solution-42
3.4.4.3 Composition of Acid Detergent Solution--42
3.4.5 In-vitro gas production----43
3.4.6 Statistical Analysis-----45
RESULTS-------46
DISCUSSION------59
CHAPTER SIX
CONCLUSION AND RECOMMENDATION--63
6.1 Conclusion------63
6.2 Recommendation-----63
REFERENCES-------64
LIST OF TABLES
Table 2.1: Chemical composition of oesophageal samples collected from sheep grazing Panicum maximum cv gatton pastures at different levels of maturity during summer, autumn or winter…………………..8
Table 2.2: Six Steps (phases) of silage fermentation and storage………………..18
Table 4.1: Characteristics of ensiled Guinea grass at different ages…………….46
Table 4.2: Coefficient of preference ensiled Guinea grass at different ages…….46
Table 4.3: chemical of composition of fresh and ensiled Guinea grass harvested at 4, 6 and 8 weeks of age………………………………….47
Table 4.4: Cell wall fraction of fresh and ensiled Guinea grass harvested at 4, 6 and 8 weeks of age…………………………………………….47
Table 4.5: Volume of gas produced at different hours of incubation by freshand ensiled Guinea grass harvested at 4, 6 and 8 weeks of age………48
Table 4.6: Post in vitro fermentation characteristics of fresh and ensiledGuinea grass harvested at 4, 6 and 8 weeks of age…………………...49
LIST OF FIGURES
Figure 1: Volume of gas produced at different hours by fresh Guinea grass harvested at 4, 6 and 8 weeks of age………………………………….49
Figure 2: Volume of gas produced at different hours by ensiled Guinea grass harvested at 4, 6 and 8 weeks of age……………………………………………………… 50
Figure 3: Volume of gas produced at different hours by fresh and ensiled Guinea grass harvested at 4, 6 and 8 weeks of age.…………………..50
Figure 4: Dry matter (DM) content of fresh and ensiled Guinea grass harvested at 4, 6 and 8 weeks of age……………………………………………..51
Figure 5: Crude protein (CP) content of fresh and ensiled Guinea grass harvestedat 4, 6 and 8 weeks of age……………………………………………..51
Figure 6: Ash content of fresh and ensiled Guinea grass harvested at 4, 6 and 8 weeks of age……………………………………………………..52
Figure 7: Organic matter (OM) content of fresh and ensiled Guinea grass harvested at 4, 6 and 8 weeks of age…………………………………..52
Figure 8: Neutral detergent fibre (NDF) content of fresh and ensiled Guinea grass harvested at 4, 6 and 8 weeks of age…………………………………..53
Figure 9: Acid detergent fibre (ADF) content of fresh and ensiled Guinea grass harvested at 4, 6 and 8 weeks of age…………………………………..53
Figure 10: Hemicellulose content of fresh and ensiled Guinea grass harvested at 4, 6 and 8 weeks of age………………………………………………54
Figure 11: Volume of methane (CH4) gas produced by fresh and ensiled Guineagrass harvested at 4, 6 and 8 weeks of age…………………………..54
Figure 12: Dry matter (DMD) content of fresh and ensiled Guinea grass harvestedat 4, 6 and 8 weeks of age……………………………………………55
Figure 13: Organic matter digestibility (OMD) content of fresh and ensiled Guinea grass harvested at 4, 6 and 8 weeks of age………………….55
Figure 14: Short chain fatty acid (SCFA) content of fresh and ensiled Guinea grass harvested at 4, 6 and 8 weeks of age………………………..56
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