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Chemical composition, health promoting potentials and storage properties of biscuits supplemented with orange peel and pulp flours

 

Table Of Contents


Chapter ONE

1.1 Introduction
1.2 Background of Study
1.3 Problem Statement
1.4 Objective of Study
1.5 Limitation of Study
1.6 Scope of Study
1.7 Significance of Study
1.8 Structure of the Research
1.9 Definition of Terms

Chapter TWO

2.1 Overview of Biscuits and Ingredients
2.2 Nutritional Value of Orange Peel and Pulp Flours
2.3 Health Benefits of Orange Peel and Pulp
2.4 Previous Studies on Biscuit Supplementation
2.5 Consumer Acceptance of Functional Foods
2.6 Formulation of Biscuits with Orange Peel and Pulp Flours
2.7 Effects of Processing on Nutritional Content
2.8 Shelf-life Studies of Biscuits
2.9 Sensory Evaluation of Biscuits
2.10 Market Trends in Functional Foods

Chapter THREE

3.1 Research Design and Methodology
3.2 Selection of Samples
3.3 Preparation of Biscuit Samples
3.4 Experimental Procedure
3.5 Data Collection Methods
3.6 Statistical Analysis Techniques
3.7 Quality Control Measures
3.8 Ethical Considerations

Chapter FOUR

4.1 Analysis of Nutritional Composition
4.2 Evaluation of Storage Properties
4.3 Comparison with Control Biscuits
4.4 Sensory Attributes of Biscuits
4.5 Consumer Feedback and Preferences
4.6 Interpretation of Results
4.7 Discussion of Findings
4.8 Implications for Future Research

Chapter FIVE

5.1 Summary of Findings
5.2 Conclusions Drawn from the Study
5.3 Recommendations for Further Research
5.4 Practical Applications of the Study
5.5 Contribution to the Field of Food Science

Project Abstract

Abstract
Biscuits are popular snack items consumed worldwide, and fortification with fruit by-products such as orange peel and pulp flours can enhance their nutritional value. This study aimed to investigate the chemical composition, health-promoting potentials, and storage properties of biscuits supplemented with orange peel and pulp flours. The orange peel and pulp flours were incorporated into the biscuit formulation at different levels (5%, 10%, and 15%), and the control biscuits were prepared without any supplementation. The chemical analysis revealed that the orange peel and pulp flours were rich sources of dietary fiber, phenolic compounds, and vitamin C. The supplemented biscuits showed a significant increase in dietary fiber content, total phenolic content, and ascorbic acid content compared to the control biscuits. The antioxidant activity of the biscuits also increased with the addition of orange peel and pulp flours, as evidenced by the DPPH scavenging activity and ferric reducing antioxidant power assays. Sensory evaluation results indicated that the biscuits supplemented with orange peel and pulp flours were well accepted by the panelists in terms of appearance, flavor, texture, and overall acceptability. The 10% supplementation level was preferred by the panelists as it provided a good balance between the orange flavor and the biscuit taste. In terms of storage properties, the supplemented biscuits showed good stability during the storage period of 30 days. There were no significant changes in the moisture content, water activity, or color parameters of the biscuits during storage. However, lipid oxidation increased slightly over time in all biscuit samples, with the supplemented biscuits exhibiting lower levels of peroxide value compared to the control biscuits. Overall, the results of this study suggest that the supplementation of biscuits with orange peel and pulp flours can enhance their nutritional value by increasing the dietary fiber, phenolic compounds, and antioxidant activity. The supplemented biscuits also showed good sensory acceptability and stability during storage. Therefore, incorporating orange peel and pulp flours into biscuits could be a promising strategy to develop functional food products with potential health benefits.

Project Overview

INTRODUCTION

            Sweet orange is one of the most important fruits in the tropical and sub-tropical regions of the world. The fruits are usually eaten fresh but are also used for making canned orange juice, frozen juice concentrate, jams, jellies among others. Orange processing industries generate huge amounts of orange peel and pulp as by products from the industrial extraction of orange juices. These peel and pulp contain among other things high levels of vitamin C, dietary fibre and flavonoids. Dietary fibre has been used for the treatment of various gastrointestinal disorders and for such possible health benefits as lowering cholesterol levels, reducing risk of colon cancer and losing weight (Friedman, 1989). Dietary fibre has also been reported to have some nutraceutical potentials (Weingartner et al., 2008). Orange peel and pulp also contain other phytochemicals such as polymethoxylated flavones (PMF) and hesperidin which have hormonal and antioxidant actions and are also involved in enzyme stimulation (Gardon, 1990).

Until just recently, analysis of food was limited to sensory and its nutritional value. However, there is growing evidence that other components of food may play an integral role in the link between food and health (Kaira, 2003). Consumers are increasingly interested in the health benefits of food and have begun to look beyond the basic nutritional benefits to the potential disease prevention and health enhancing compounds contained in many foods (Hasler, 2003).

Nutraceutical, a term combining the words β€œnutrition and pharmaceutical,” is a food with a medical health benefit, including prevention and treatment of disease. This definition includes any substance that may be considered a food or part of a food and provides medical or health benefits, including the prevention and treatment of disease. Such products may range from isolated nutrients, dietary supplements and diets to genetically engineered foods, herbal products and processed foods such as cereals, soups, beverages among others. (Defelice, 1994). Examples of foods with nutraceutical values are broccoli (Sulforphane), which may help in the prevention of cancer, resveratrol from red grape products as an antioxidant, flavonoids in citrus, tea, wine and dark chocolate (Weingartner et al., 2008). Many botanical and herbal extracts such as ginseng, garlic oil etc have been developed as nutraceuticals. The use of nutraceuticals to accomplish desirable therapeutic outcomes with reduced side effects as compared with other therapeutic agents has met with great success (Whitman, 2001; Nelson, 1999). The peel and pulp of orange fruits have been noted to contain some bioactive substances believed to possess nutraceutical potentials (Kootstra, 1994). Thus, the incorporation of orange peel and pulp into wheat flour for the production of biscuit would enhance greater utilization of phytochemicals in Nigerian diets.

Biscuit is a confectionary, dried to very low moisture content (Okaka, 1997). Biscuit is a snack food which can be eaten in-between meals or at any time of the day and by any age bracket. An increasing proportion of the household food budget in Nigeria is spent on snacks in which convenience and quality are perceived as most important (Lasekan and Akintola, 2002). Biscuits contain fat (18.5 %), carbohydrate (78.23%), ash (1.0 %) and salt (0.85 %) (Okeagu, 2001). They are generally characterized by a low moisture content (Okaka, 1997), The shelf life is several months under correct storage conditions (Ihekoronye, 1999). However, biscuits must be packaged in containers which prevent moisture uptake (Okaka, 1997).

 

1.1 STATEMENT OF PROBLEM

The juice obtained from orange fruits is widely consumed while the pulp and peel are discarded. The amount of peel and pulp obtained from citrus fruit processing accounts for 50% of the original amount of the whole fruit (Chon and Chon, 1997). These Peel and Pulp contain some bioactive substances believed to have nutraceutical potentials.

Biscuit consumption among children and adult is high in Nigeria. Orange peel and pulp could be incorporated into wheat for biscuit production because of their phytochemical content. However, the storage properties and nutraceutical potential of biscuit containing orange peel and pulp need to be assessed.

 

1.2 BROAD OBJECTIVE OF THE STUDY  

The broad objective of the study was to determine the chemical composition, health promoting potentials and storage stability of biscuits supplemented with orange peel and pulp flours.

 

1.3 SPECIFIC OBJECTIVES

1) To produce biscuits from blends of wheat and orange peel and pulp flours.

2) To determine the chemical composition of the biscuits.

3) To evaluate the health promoting potentials of the biscuits using bio-assay study.

4) To determine the storage stability of the biscuits.


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