Project Abstract

Abstract
This study aimed to compare the protein contents of breadfruit, brown beans, and soybeans using various analytical methods. The protein content of food products is crucial for assessing their nutritional value and suitability for different dietary requirements. Breadfruit, brown beans, and soybeans are all rich sources of plant-based proteins, making them popular choices for vegetarians, vegans, and individuals looking to incorporate more plant proteins into their diets. In this comparative study, samples of breadfruit, brown beans, and soybeans were collected and analyzed for their protein contents using the Kjeldahl method, which is a widely accepted technique for determining protein levels in food samples. Additionally, the samples were also analyzed using the Bradford assay and the Biuret method to compare the results obtained from different analytical approaches. The results of the study showed that soybeans had the highest protein content among the three samples, followed by brown beans and breadfruit. The protein content of soybeans was found to be significantly higher compared to breadfruit and brown beans, which is consistent with existing literature on the protein composition of these food sources. The Kjeldahl method, Bradford assay, and Biuret method all produced consistent results, indicating the reliability of these analytical techniques for protein determination in plant-based food products. The findings of this study have important implications for individuals seeking to optimize their protein intake through plant-based sources. Soybeans emerged as a superior protein source compared to breadfruit and brown beans, based on the results obtained from the analytical methods employed in this study. This information can be valuable for nutritionists, dietitians, and individuals looking to make informed choices about their dietary protein sources. Overall, this comparative analysis of protein contents in breadfruit, brown beans, and soybeans provides valuable insights into the nutritional composition of these plant-based foods. Further research can explore additional factors such as amino acid profiles, digestibility, and bioavailability of proteins in these food sources to gain a more comprehensive understanding of their nutritional value. The findings of this study contribute to the existing body of knowledge on plant-based proteins and can inform dietary recommendations for individuals seeking to enhance their protein intake through plant sources.

Project Overview

1.1 Background of the Study

Proteins
are essential nutrients for the human body (Hermann, 2002). They are one of the
building block of the body tissue, and also serve as a fuel source. As a fuel,
protein contain 4kcal (17kj) per gram, just like carbohydrates and unlike
lipids, which contain 9kcal (37kj) per gram. The most important aspect and
defining characteristics of protein from a nutritional stand point is its amino
acid composition (Laurence, 2000).

Proteins
are polymer chains made of amino acids linked together by peptide bonds. During
human digestion, proteins are broken down in the stomach to smaller polypeptide
chain via hydrochloric acid and protease actions. This is crucial for the
synthesis of the essential amino acids that cannot be biosynthesized by the
body (Genton, 2010). There are nine essential amino acids which humans must
obtain from their diet in order to prevent protein-energy malnutrition. They
are phenylalanine, valine, lysine, leucine, threonine, tryptophan, methionine,
isoleucine and histidine (Laurence, 2000). There are five dispensable amino
acids which humans are able to synthesize in the body. These five are alanine,
aspartic acid, sernine, asparagines and glutamic acid. There are six
conditionally essential amino acids whose synthesis can be limited under
special pathophysiological conditions, such as prematurity in the infant or
individuals in severe catabolic distress (Laurence, 2000). These six are
argnine, cysteine, glycine, glutamine, proline and tryrosine (Laurence, 2000).
Sources of protein include grains, legumes and nuts, as well as animal sources
such as meats, dairy products, fish and eggs (Young, 1994).

African
breadfruit (Treculia Africana Decne) belongs
to the mulberry family. Moracceae, which is of African origin but now grown in
the most tropical and sub-tropical countries (Agu and Nwabueze, 2007). African
breadfruit or wild jack fruit in some areas, is a neglected and under exploited
tropical tree (Osuji and Owei, 2010).

According
to Okonkwo and Ubani (2012), it is a common forest tree called various names
among different tribes in Nigeria, such as “Ukwa” (Igbo), “afon” (Yoruba),
“eyo” (Igala), “barafuta” (Hausa), “Ize” (Benin) and “edikang” (Efik). The tree
crop is widely grown in the southern state of Nigeria where it serves as low
cost meat substituent for poor families in some communities (Badifu and Akuba,
2001; Ugwu, et al, 2001). the plant produced large, usually round, compound
fruit covered with pointed outgrowths and the seeds are buried in the spongy
pulp of the fruits (Nwokolo, 1996). the seeds are seldom eaten raw but can be
baked, roasted or fried before consumption, or they can be ground into flour in
bakery products (Agu et al, 2007; Ijeh et al, 2010). African breadfruit seeds
are highly nutritious and constitute a cheap source of vitamins, minerals,
proteins, carbohydrates and fats.

Brown
beans (Phaseolus Vulgaris) is a
herbaceous annual plant grown worldwide for its edible dry seeds (Known as just
‘Beans”) or unripe fruit (Green beans). It’s leaf is also occasionally used as
a vegetable and the straw as fodder. It’s botanical classification, along with
other phaseolus species, is as a member of the legume family fabaceae, most of
whose members acquire the nitrogen they require through association with
rhizoidal, a species of nitrogen-fixing bacteria (Edet, 1982). Beans are grown
in every continent except Antarctica. Brazil and India are the largest
producers of dry beans, while china produces by far, the largest quantity of
brown beans. Worldwide, 23 million tones of dry common beans and 17.1 billion
tones of green were grown in 2010 (Philips, 2010). Similar to other beans, the
brown beans is high in starch, protein and dietary fiber, and is an excellent
source of iron, selenium, potassium, molybdenum, thiamine, vitamin B6
and folate (Paul, 1998) .

The
soybean (Glycine max (L.) Merrill
family Leguminosae, subfamily Papilionoidae) originated in Eastern Asia,
probably in north and central china. It is believed that cultivated varieties were
introduced into Korea and later Japan some 2000 years ago. Soybeans have been grown
as food crop for thousands of years in China and other countries of East and South
East Asia and constitute to this day, an important component of the traditional
popular diet in these regions (William, 2003). Although the U.S.A and Brazil
account today for the most of the soybean production of the world, the
introduction of this crop to Western agriculture is quite recent. Soybeans are
primarily, an industrial crop, cultivated for oil protein. Despite the
relatively low oil content of the seed (about 20% on moisture-free basis),
Soybeans are the largest single source of edible oil and account for roughly
50% of total oil seed production of the world (Singh, Nelson and Chung, 2008).
With each ton of crude soybean oil, approximately 4.5 tons of soybean oil meal
with a protein content of about 44% are produced. For each ton of soybeans
processed, the commercial value of the meal obtained usually exceeds that of
the oil. Thus, soybean oil meal cannot be considered by-product of the oil manufacture.
The soybean is, in this respect, an exception among oil seed (Shurtleff; Steenhuis
and Spiers, 2013). It can be calculated that the quality of protein in the
yearly world production of soybeans, if it could be totally and directly
utilized for human consumption would be sufficient for providing roughly one
third of the global need for protein (William, 2003). This makes the soybeans
one of the largest potential source of dietary protein. However, the bulk of
soybean oil meal is used in animal feed for the production of meat and eggs.
Despite considerable public and commercial interest in soybean products as
food, the proportion of soybean protein consumed directly in human nutrition is
still relatively small (Smith, 1972).

1.2   Statement of Problem

It
has been scientifically proven that every variety of beans is rich in protein
and contains such amount of carbohydrates that is good for diabetic patients.
This not the case with African breadfruit, while some say that it is highly protein
ones other say it contains mostly carbohydrates. This controversy has created
confusion to many especially diabetic patients who are cross road whether to
keep eating it or not. This research is therefore aimed at setting the
controversy.

1.3   Objective of the Study

The
general objective of this present work is to determine and compare the protein
content of breadfruit, brown beans and soybean. The specific objectives are as
follows;

·                
To
determine the protein content of breadfruit.

·                
To
determine the protein content of brown beans.

·                
To determine the protein content of
soybeans.

·                
Comparison of protein content of
breadfruit, brown beans and soybeans.

1.4   Significance of the Study

The
research will be beneficial to the following;

·                
Diabetic patients

·                
Dieticians

·                
People in the health sector

·                
Vegetarians and

·                
The general public

1.5   Scope of the Study

This
research work is limited to the comparative determination of protein content of
breadfruit, brown beans and soybeans.

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