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Antibacterial activity of honey on staphylococcus aureus escherichia coli and streptococcus pyogen isolated from wound

 

Table Of Contents


Chapter ONE

1.1 Introduction
1.2 Background of Study
1.3 Problem Statement
1.4 Objective of Study
1.5 Limitation of Study
1.6 Scope of Study
1.7 Significance of Study
1.8 Structure of the Research
1.9 Definition of Terms

Chapter TWO

2.1 Overview of Antibacterial Activity
2.2 Properties of Honey Relevant to Antibacterial Activity
2.3 Staphylococcus aureus: Characteristics and Antibiotic Resistance
2.4 Escherichia coli: Characteristics and Pathogenicity
2.5 Streptococcus pyogen: Pathogenicity and Antibiotic Resistance
2.6 Previous Studies on Honey's Antibacterial Activity
2.7 Comparison with Other Natural Antibacterial Agents
2.8 Mechanisms of Honey's Antibacterial Action
2.9 Bioactive Components in Honey
2.10 Future Research Directions

Chapter THREE

3.1 Research Design and Methodology
3.2 Selection of Honey Samples
3.3 Isolation and Culturing of Bacterial Strains
3.4 Antibacterial Susceptibility Testing Methods
3.5 Experimental Setup for Antibacterial Testing
3.6 Data Collection and Analysis
3.7 Ethical Considerations
3.8 Statistical Analysis Techniques

Chapter FOUR

4.1 Overview of Research Findings
4.2 Antibacterial Activity of Different Honey Samples
4.3 Comparison of Antibacterial Efficacy on Staphylococcus aureus
4.4 Comparison of Antibacterial Efficacy on Escherichia coli
4.5 Comparison of Antibacterial Efficacy on Streptococcus pyogen
4.6 Relationship between Honey Properties and Antibacterial Activity
4.7 Discussion on Mechanisms of Action
4.8 Implications for Wound Care

Chapter FIVE

5.1 Summary of Findings
5.2 Conclusion
5.3 Recommendations for Further Research
5.4 Practical Applications and Future Implications
5.5 Reflection on Research Process

Thesis Abstract

Antibacterial activity of honey obtained from two different locations in Enugu State (Nsukka & Ugwuaji) Nigeria on Staphylococcus aureus, Escherichia coli and Streptococcus pyogens isolated from wound was studied. Agar well diffusion method was used to determine the antibacterial activity of the honey on the test microorganisms. The result revealed that the two honey samples have heavy antibacterial activities against the test organisms and zones of inhibition were obtained showing high antibacterial activity. The antibacterial activity increased with increase in the concentrations and honey from Nsukka produced a high antibacterial activity (clearer zone) on staphylococcus aureus and Escherichia coli at all concentration and moderately for streptococcus pyogens. The use of honey as a therapeutic substance has been rediscovered by the medical profession on more recent times, and it is gaining acceptance as an antibacterial agent for the treatment of ulcers and bed sores, and other infections resulting from burns and wounds.

Thesis Overview

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