Home / Medical Laboratory Science / SUSCEPTIBILITIES OF SALMONELLA TYPHI AND OTHER BACTERIAL PATHOGENS TO ANTIBIOTICS AND HOT AQUEOUS EXTRACT OF HIBISCUS SABDARIFFA

SUSCEPTIBILITIES OF SALMONELLA TYPHI AND OTHER BACTERIAL PATHOGENS TO ANTIBIOTICS AND HOT AQUEOUS EXTRACT OF HIBISCUS SABDARIFFA

 

Table Of Contents


Chapter ONE

1.1 Introduction
1.2 Background of study
1.3 Problem Statement
1.4 Objective of study
1.5 Limitation of study
1.6 Scope of study
1.7 Significance of study
1.8 Structure of the research
1.9 Definition of terms

Chapter TWO

2.1 Antibiotic resistance in bacterial pathogens
2.2 Mechanisms of antibiotic resistance
2.3 Importance of studying antibiotic susceptibility
2.4 Hibiscus sabdariffa as a natural remedy
2.5 Antibacterial properties of Hibiscus sabdariffa
2.6 Previous research on antibiotic susceptibility
2.7 Current trends in antibiotic resistance
2.8 Impact of antibiotic misuse
2.9 Alternatives to antibiotics
2.10 Future directions in antibiotic research

Chapter THREE

3.1 Research design
3.2 Sampling methods
3.3 Data collection techniques
3.4 Data analysis procedures
3.5 Ethical considerations
3.6 Research limitations
3.7 Data validity and reliability
3.8 Research assumptions

Chapter FOUR

4.1 Overview of research findings
4.2 Antibiotic susceptibility testing results
4.3 Comparison of Hibiscus sabdariffa extract with antibiotics
4.4 Factors influencing bacterial susceptibility
4.5 Interpretation of findings
4.6 Discussion on implications of results
4.7 Recommendations for future research
4.8 Limitations of the study

Chapter FIVE

5.1 Summary of research findings
5.2 Conclusion
5.3 Contributions to the field
5.4 Implications for public health
5.5 Recommendations for practice
5.6 Suggestions for further research
5.7 Final thoughts

Thesis Abstract

The susceptibilities of Salmonella typhi and other pathogens to antibiotics and hot aqueous extract of Hibiscus sabdariffa were investigated using agar diffusion and agar well diffusion methods respectively. Salmonella typhi was sensitive to ampicillin, cetriaxone, ciprofloxacin, gentamycin of ofloxacin and perfloxacin. Nitrofurantoin, ampicillin, clarithomycin and augumentin are resistant. Escherichia coli, Klebsiella spp, and Staphylococcus aureus were sensitive to 50%, 70% and 60% of the antibiotics respectively.Pseudomonas aeruginosa was resistant to all antibiotics. Hibiscus sabdariffa extract (0.6g in 6ml of sterile distilled water) was active against S. typhi at concentrations of 100mg/ml, 50mg/ml and 25mg/ml (inhibitions zone diameter IZDs = 23mm, 20mm and 16mm respectively). Staphylococcus aureus was susceptible to 100mg/ml, 50mg/ml, 25mg/ml and 12.5mg/ml of the extract with IZDs of 29mm, 18mm, 17mm and 14mm respectively. Klebsiella spp was susceptible to concentrations of 25mg/ml and 12.5mg/ml of the extract with IZDs of 15mm and 10mm respectively. Escherichia coli and Pseudomonasaeruginosa were resistant to all the concentrations of H. sabdariffa extract. It is therefore imperative to note that the use of medicinal plants is recommended to the Government and Industry.

Thesis Overview

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