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Effect of different carbon sources on the growth of antimicrobial producing bacillus species in citrillus vugaris.

 

Table Of Contents


Chapter ONE

1.1 Introduction
1.2 Background of Study
1.3 Problem Statement
1.4 Objective of Study
1.5 Limitation of Study
1.6 Scope of Study
1.7 Significance of Study
1.8 Structure of the Research
1.9 Definition of Terms

Chapter TWO

2.1 Overview of Carbon Sources
2.2 Growth Factors in Bacillus Species
2.3 Antimicrobial Production in Bacillus
2.4 Citrullus Vulgaris as a Host Plant
2.5 Effect of Carbon Sources on Microbial Growth
2.6 Influence of Carbon Sources on Antimicrobial Production
2.7 Interactions between Bacillus Species and Citrullus Vulgaris
2.8 Importance of Studying Carbon Sources in Microbial Growth
2.9 Comparative Analysis of Different Carbon Sources
2.10 Research Gaps in Carbon Source Studies

Chapter THREE

3.1 Research Design
3.2 Sampling Methods
3.3 Data Collection Techniques
3.4 Experimental Setup
3.5 Statistical Analysis Methods
3.6 Variables and Controls
3.7 Ethical Considerations
3.8 Data Interpretation Techniques

Chapter FOUR

4.1 Analysis of Growth Patterns
4.2 Antimicrobial Production Levels
4.3 Impact of Carbon Sources on Bacillus Growth
4.4 Relationship Between Carbon Sources and Antimicrobial Activity
4.5 Comparison of Growth in Different Carbon Sources
4.6 Influence of Citrullus Vulgaris on Bacillus Species
4.7 Interpretation of Experimental Results
4.8 Discussion on the Findings

Chapter FIVE

5.1 Summary of Research Findings
5.2 Conclusion
5.3 Recommendations for Future Studies
5.4 Practical Implications
5.5 Contribution to the Field
5.6 Limitations of the Study
5.7 Final Thoughts
5.8 References

Thesis Abstract

Effect of different carbon sources on the growth of antimicrobial producing Bacillus species in ogiri-egusi sold in Ogbeta, Orie, Artisian, Kenyatta, Enugu State were evaluated using standard microbiological and analytical methods. 12 bacteria were isolated and identified such as Bacillus subtilis,(33.33), Bacillus pumulis (41.66), Bacillus licheniformis (25) were identified based on their colony biochemical characteristics.The isolates of the highest occurrences is bacillus pumilus. Agar well diffusion method was used to determine the antimicrobial activity against two pathogenic organisms, Escherichia coli, Staphylococcus aureus. Bacillus subtilis and Bacillus pumulis, gave a zone of inhibition Escherichia coli 0.6mm and 0.8mm after 24 hours of incubation also carbon sources of 5% and 10% sucrose, Glucose and soluble starch were used to check their different carbon sources on the isolates using the spectrophotometer.5% had the highest optimum growth of 1.400 for glucose 1.545 for sucrose,2.376 for soluble starch which can be added in maximum quantity to a medium to produce antibiotics.

Thesis Overview

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