The phytochemical constituents of methanolic extract of ocimum gratissimum
Table Of Contents
Chapter ONE
1.0 Introduction
1.1 Aim of Study
1.2 Literature Review
1.3 Phytochemicals
1.3.1 Alkaloids
1.3.2 Flavonoids
1.3.2.1 Antioxidant activities
1.3.2.2 Antimicrobial activities
1.3.2.3 Antibacterial activities
1.3.2.3 Antiatherosclerotic effects
1.3.3 Saponins
1.3.4 Tannins
Chapter TWO
2.0 Materials and Methods
2.1 Materials Used
2.2 Collection and Identification of samples
2.3 Sample preparation
2.3.1 Drying of the leaves
2.3.2 Pulverisation of the leaves
2.3.3 Extraction Procedure
2.3.4 Filtration Process
2.3.5 Concentration Process
2.4 Phytochemical Analysis
2.4.1 Oualitative Analysis
2.4.1.1 Test for Alkaloids
2.4.1.2 Test for Phytosterol
2.4.1.3 Test for Flavonoids
2.4.1.4 Test for Saponin
2.4.1.5 Test for Phenol and Tannin
2.4.2 Ouantitative Analysis
2.4.2.1 Determination of flavonoid content
2.4.2.2 Determination of tannins content
2.4.2.3 Determination of Saponins content
2.4.2.4 Determination of flavonoid content
2.4.2.5 Determination of Phenolic content
2.4.2.6 Determination of Reducing Power
Chapter THREE
3.0 Results
Chapter FOUR
4.0 Discussion and Conclusion
4.1 Discussion
4.2 Conclusion
References
LIST OF TABLES
Table 1 – Showing the qualitative analysis results of phytochemical constituents of Ocimum grattissimum
Table 2 – Showing the quantitative analysis of phytochemical constituents of Ocimum grattissimum
Table 3 – Showing the flavonoids determination
Table 4 – Showing the tannin determination
Table 5 – Showing the total phenol determination
Table 6 – Showing the ascorbic acid absorbance
Table 7 – Bioactivity of compounds detected through GC-MS screening of methanolic extract of scent leaves
LIST OF FIGURES
Figxure 1 – Graph showing the flavonoids determination
Figure 2 – Graph showing the tannin determination
Figure 3 – Graph showing the total Phenol determination
Figure 4 – Graph showing the reducing standard curve.
Thesis Abstract
AbstractOcimum gratissimum, commonly known as "Scent leaf," is a plant widely used in traditional medicine for its various health benefits. In this study, we aimed to investigate the phytochemical constituents of the methanolic extract of Ocimum gratissimum. The plant material was collected, dried, and ground to obtain a fine powder, which was then subjected to extraction with methanol. The methanolic extract was then analyzed for its phytochemical constituents using standard qualitative tests. The phytochemical screening of the methanolic extract of Ocimum gratissimum revealed the presence of various bioactive compounds, including alkaloids, flavonoids, tannins, saponins, glycosides, and phenols. Alkaloids are known for their pharmacological activities, including analgesic and anti-inflammatory properties. Flavonoids possess antioxidant and anti-inflammatory properties, while tannins have antimicrobial and antioxidant activities. Saponins exhibit anti-inflammatory and immunomodulatory effects, and glycosides have cardioprotective and antioxidant properties. Phenols are known for their antioxidant and anti-inflammatory activities. The presence of these phytochemical constituents in the methanolic extract of Ocimum gratissimum suggests its potential therapeutic benefits. Alkaloids, flavonoids, tannins, saponins, glycosides, and phenols are known to exhibit a wide range of biological activities, including antioxidant, anti-inflammatory, antimicrobial, and immunomodulatory effects. These bioactive compounds make Ocimum gratissimum a promising candidate for further pharmacological investigations. Further studies are warranted to isolate and identify the specific phytochemical compounds present in the methanolic extract of Ocimum gratissimum. Additionally, in vitro and in vivo studies are needed to evaluate the pharmacological activities and potential therapeutic applications of these bioactive compounds. Understanding the phytochemical constituents of Ocimum gratissimum can provide valuable insights into its medicinal properties and help in the development of new therapeutic agents for various health conditions. In conclusion, the methanolic extract of Ocimum gratissimum contains a diverse array of phytochemical constituents with potential pharmacological activities. Further research is needed to elucidate the specific bioactive compounds responsible for these effects and to explore the therapeutic potential of this plant in the treatment of various diseases.
Thesis Overview
Blazingprojects Mobile App
📚 Over 50,000 Research Thesis
📱 100% Offline: No internet needed
📝 Over 98 Departments
🔍 Thesis-to-Journal Publication
🎓 Undergraduate/Postgraduate Thesis
📥 Instant Whatsapp/Email Delivery
Related Research
Assessing the effectiveness of multimedia simulations in teaching cellular respirat...
...
Exploring the impact of outdoor fieldwork on student attitudes towards biology....
...
Investigating the use of concept mapping in teaching biological classification....
...
Analyzing the influence of cultural diversity on biology education....
...
Assessing the impact of cooperative learning on student understanding of genetics....
...
Investigating the effectiveness of online quizzes in promoting biology knowledge re...
...
Exploring the use of storytelling in teaching ecological concepts....
...
Analyzing the impact of teacher-student relationships on student achievement in biol...
...
Investigating the role of metacognitive strategies in biology learning....
...
