<p>
</p><p><strong>
Chapter ONE
</strong></p><p>Introduction</p><p>Aim of the Study</p><p>1.0 Literature Review</p><p>1.1 Macro and Micro elements</p><p>1.2 Phytochemicals</p><p>
Chapter TWO
</p><p>2.0 Materials and Methods</p><p>2.1 Materials Used</p><p>2.2 Reagents Used</p><p>2.3 Identification and Preparation of plant materials</p><p>2.4 Sample preparation</p><p>2.5 Phytochemical analysis</p><p>2.5.1 Qualitative analysis</p><p>2.5.1.1 Test for Alkaloids</p><p>2.5.1.2 Test for Glycosides and Steroids</p><p>2.5.1.3 Test for Terpenoids</p><p>2.5.1.4 Test for Saponin</p><p>2.5.1.5 Test for Phenol</p><p>2.5.1.6 Test for Tannins</p><p>2.5.2 Quantitative analysis</p><p>2.5.2.1 Estimation of total flavonoids</p><p>2.5.2.2 Preparation of Quercetin</p><p>2.5.2.3 Determination of tannin concentration</p><p>2.5.2.4 Preparation of tannic acid standard curve</p><p>2.5.2.5 Determination of Saponin concentration</p><p>2.5.2.6 Determination of Alkaloid concentration</p><p>2.5.2.7 Determination of total phenolic content</p><p>2.5.2.8 Preparation of standard for gallic acid</p><p>2.5.2.9 Determination of ferric reducing power</p><p>2.5.2.10 Gas chromatography, mass spectroscopic analysis</p><p>
Chapter THREE
</p><p>3.0 Results</p><p>
Chapter FOUR
</p><p>4.0 Discussion and Conclusion</p><p>4.1 Discussion</p><p>4.2 Conclusion</p><p>4.3 Recommendation</p><p>References</p><p>Appendix</p>
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