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Isolation and identification of microoragnisms in cassava mill effluent in the soil

 

Table Of Contents


Chapter ONE

1.1 Introduction
1.2 Background of Study
1.3 Problem Statement
1.4 Objective of Study
1.5 Limitation of Study
1.6 Scope of Study
1.7 Significance of Study
1.8 Structure of the Research
1.9 Definition of Terms

Chapter TWO

2.1 Introduction to Literature Review
2.2 Theoretical Framework
2.3 Conceptual Framework
2.4 Previous Studies on Microorganisms in Cassava Mill Effluent
2.5 Impact of Microorganisms on Soil Ecosystem
2.6 Methods for Isolation and Identification of Microorganisms
2.7 Role of Microorganisms in Soil Health
2.8 Environmental Implications of Microorganisms in Cassava Mill Effluent
2.9 Technological Approaches for Microorganism Analysis
2.10 Summary of Literature Review

Chapter THREE

3.1 Research Methodology Overview
3.2 Research Design
3.3 Sampling Techniques
3.4 Data Collection Methods
3.5 Data Analysis Procedures
3.6 Laboratory Techniques for Microorganism Isolation
3.7 Identification Methods for Microorganisms
3.8 Quality Control Measures

Chapter FOUR

4.1 Introduction to Discussion of Findings
4.2 Microorganisms Identified in Cassava Mill Effluent
4.3 Microorganisms Found in Soil Samples
4.4 Comparison of Microbial Diversity in Cassava Mill Effluent and Soil
4.5 Effects of Microorganisms on Soil Nutrient Cycling
4.6 Implications for Agricultural Practices
4.7 Environmental Impact Assessment
4.8 Recommendations for Future Research

Chapter FIVE

5.1 Conclusion and Summary of Research
5.2 Summary of Findings
5.3 Implications for Agriculture and Environmental Management
5.4 Contributions to Scientific Knowledge
5.5 Recommendations for Policy and Practice

Thesis Abstract

The Isolation and identification of microorganisms in cassava mill effluent in the soil in Orji, Imo State, Nigeria were carried out, soil samples were collected from site polluted with cassava mill effluent and also from adjacent site not polluted with cassava effluent to serve as control. The overall population of microorganisms in cassava mill effluent was determined. Isolation and characterization of succession of microorganism in cassava mill effluent was studied microbial count, Bacterial counts and fungal counts were also determined using a pour plate method. The microbial count ranges from 2.2 X 105 – 2.0 X 105 for THBC, 1.1 X 105 – 1.2 X 105 for TCC and the TFC ranges from 1.2 X 105-1.5 X 105. The bacteria isolated were Klebsiella spp., Escherichia coli , Pseudomonas spp., Staphylococcus aureus, and Streptococcus spp. while the fugal isolates include Penicillium spp., Aspergillus spp., mucor spp., and yeast cells Candida spp. the cassava mill effluent should be treated before discharge into the environment to prevent pollution.

Thesis Overview

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