Isolation, identification and antibiogram of staphylococcus aureus isolated from cow meat
Table Of Contents
Chapter ONE
1.1 Introduction1.2 Background of study
1.3 Problem Statement
1.4 Objective of study
1.5 Limitation of study
1.6 Scope of study
1.7 Significance of study
1.8 Structure of the research
1.9 Definition of terms
Chapter TWO
2.1 Overview of Staphylococcus aureus2.2 History of Staphylococcus aureus
2.3 Morphology and characteristics
2.4 Pathogenesis and diseases caused
2.5 Transmission and control measures
2.6 Antibiotic resistance mechanisms
2.7 Detection and identification methods
2.8 Epidemiology of Staphylococcus aureus
2.9 Foodborne transmission of Staphylococcus aureus
2.10 Impact on public health
Chapter THREE
3.1 Research design3.2 Sampling techniques
3.3 Data collection methods
3.4 Laboratory procedures
3.5 Data analysis techniques
3.6 Ethical considerations
3.7 Research limitations
3.8 Research validity and reliability
Chapter FOUR
4.1 Descriptive analysis of Staphylococcus aureus isolates4.2 Antibiotic susceptibility testing results
4.3 Comparison with existing literature
4.4 Factors influencing antibiotic resistance
4.5 Discussion on implications for public health
4.6 Recommendations for future research
4.7 Limitations of the study
4.8 Conclusion of the findings
Chapter FIVE
5.1 Summary of findings5.2 Conclusion
5.3 Practical implications
5.4 Contribution to knowledge
5.5 Recommendations for practice
5.6 Recommendations for policy
5.7 Suggestions for further research
Thesis Abstract
The study was carried out with aim to isolate Staphylococcus aureus from cow meat and determine the antibiogram pattern of S. aureus. Three samples of cow meat from three different locations (Gwagwalada market, Abattoir market and Kasuan Dare) in Gwagwalada were collected to isolate S. aureus from the samples. The organisms isolated were Salmonella spp, Escherichia coli, Staphylococcus aureus and Listeria monocytogenes. Several biochemical tests were carried out to identify, the S. aureus isolates like Catalse test (positive), Manitol test (positive), Coagulase test (positive) and Oxidase test (negative). The antibiogram pattern of S. aureus was evaluated using commercially prepared disk. The antibiotics tested were Pefloxacin (Pef), Streptomycin (S), Ampiclox (Apx), Amoxacilin (Am) and Ciprofloxacin. The pattern indicated that the overall S. aureus were resistant to Pef, S, Cpx and less resistant to Am and Apx. Results clearly suggested a possibility of potential public health threat of S. aureus resulting from contamination of cow meat with pathogenic bacteria which is mainly due to unhygienic processing, handling, and unhygienic environment.
Thesis Overview
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