Cover page
Title page
Certification
Dedication
Acknowledgement
Abstract
Organization of the work
Table of Contents
Chapter ONE
1.0 INTRODUCTION 1 - 2
Chapter TWO
2.0 LITERATURE REVIEW 3 - 6
2.1 PURPOSE OF STUDY 6
Chapter THREE
3.0 MATERIALS AND METHODS 7
3.1 STERILIZATION OF MATERIALS 7
3.2 COLLECTION OF SAMPLE 7
3.3 PREPARATION OF MEDIA 7 - 8
3.4 SERIAL DILUTION 8
3.5 INOCULATION OF AGAR 8
3.6 SUB-CULTURING OF MICROBIAL GROWTH 8
3.7 MAINTENANCE OF PURE CULTURE 8
3.8 CHARACTERIZATION OF FUNGAL ISOLATES 9
3.9 CHARACTERIZATION OF BACTERIAL ISOLATES 9
3.9.0 GRAM STAINING 9
3.9.1 SPORE STAINING 10
3.9.2 COAGULASE TEST 10
3.9.3 OXIDASE TEST 10
3.9.4 CATALASE TEST 10
3.9.5 MOTILITY TEST 10 - 11
3.9.6 IDENTIFICATION OF FUNGAL ISOLATES 11
3.9.7 IDENTIFICATION OF BACTERIAL ISOLATES 11
Chapter FOUR
4.0 RESULTS AND DISCUSSION 12 - 15
Chapter FIVE
5.0 CONCLUSION AND RECOMMENDATION 16 - 17
REFERENCES 18
Thesis Overview
1.0 INTRODUCTIONWater is the one of
the most common substances known and it is good solvent for many
substances, water occur at room temperature as clear, colourless,
tasteless liquid which freezing into ice at zero degree centigrade and
boil at 1000c. it is essential for life on earth.According
to World Health Organization (WHO) in 1967 is the most important need
of living things, it is second only to the air we breath, in fact some
scientist believe that all life began in water. Also water is a molecule
that consist of two hydrogen atoms and one oxygen atom, when these
atoms are heated to a high temperature they join to for water. It occurs
in three natural states solid, liquid and gaseous states. Water and ice
covered about 75% of the earth surface and water vapour is an important
constituent of the atmosphere (Ababio 1985) It exists under major two
types which include:.
Surface Water: They include the streams, lakes, and shallow wells. The air through which the rain passes may contaminate the water.
Ground Water: They originate
from
deep well and subterraneous springs. This is virtually free of bacteria
due to filtering action of soil deep sand and rock. However, it may be
come contaminated when it flows along the channels.Water in
Microbiology can be contaminated when it contains a chemical or
biological poison or an infectious agent. These condition also apply to
water which is polluted except that the agent or poison is often obvious
and water carries an unpleasant taste or appearance. (Encyclopedia
America 1988). Portability refers to the drinkability of
water when it is fit for drinking, when unportable it is unfit for
consumption due to some contaminant or pollutant (Earnet 1974) other
sources of contamination are various types of establishments and
agricultural farm etc. Possible sources of microbial contamination of a
body of water are soil and agricultural run-off, farm animals,
industrial waste, discharges from sewage treatment plants and storm
water run-off from urban areas. Contamination by sewage or
human excrement and pollution by animals causes the greatest danger
associated with drinking water in most developing countries. So it is
necessary to examine the quality of water before consumption.Monitoring
and detection of indicator and disease causing microorganisms are a
major part of sanitary microbiology. Bacteria from the intestinal tract
generally do not survive in the aquatic environment. They are under
physiological stress and gradually lose their ability to form colonies
on differential and selective media (Prescott 7th Edition).