Home / Biochemistry / Effect of different carbon sources on the growth of antimicrobial producing bacillus species in citrillus vugaris.

Effect of different carbon sources on the growth of antimicrobial producing bacillus species in citrillus vugaris.

 

Table Of Contents


Chapter ONE

1.1 Introduction
1.2 Background of Study
1.3 Problem Statement
1.4 Objective of Study
1.5 Limitation of Study
1.6 Scope of Study
1.7 Significance of Study
1.8 Structure of the Research
1.9 Definition of Terms

Chapter TWO

2.1 Overview of Antimicrobial Producing Bacillus Species
2.2 Carbon Sources and Microbial Growth
2.3 Citrullus Vulgaris as a Host Plant
2.4 Relationship between Carbon Sources and Antimicrobial Production
2.5 Previous Studies on Bacillus Species and Carbon Sources
2.6 Impact of Different Carbon Sources on Bacillus Growth
2.7 Factors Influencing Antimicrobial Production
2.8 Importance of Studying Carbon Sources in Microbial Growth
2.9 Methods for Evaluating Bacillus Growth
2.10 Role of Carbon Sources in Sustainable Agriculture

Chapter THREE

3.1 Research Design and Methodology Selection
3.2 Sampling Techniques and Sample Size Determination
3.3 Data Collection Methods
3.4 Experimental Setup and Procedures
3.5 Data Analysis Techniques
3.6 Quality Control Measures
3.7 Ethical Considerations in Research
3.8 Limitations of the Research Methodology

Chapter FOUR

4.1 Growth Patterns of Antimicrobial Producing Bacillus Species
4.2 Effects of Different Carbon Sources on Bacillus Growth
4.3 Comparison of Antimicrobial Production in Various Carbon Sources
4.4 Influence of Citrullus Vulgaris on Bacillus Growth
4.5 Factors Affecting Antimicrobial Production
4.6 Statistical Analysis of Data
4.7 Discussion on the Findings
4.8 Implications of the Results

Chapter FIVE

5.1 Summary of Findings
5.2 Conclusion
5.3 Recommendations for Future Research
5.4 Practical Applications of the Study
5.5 Contribution to the Field

Thesis Abstract

Effect of different carbon sources on the growth of antimicrobial producing Bacillus species in ogiri-egusi sold in Ogbeta, Orie, Artisian, Kenyatta, Enugu State were evaluated using standard microbiological and analytical methods. 12 bacteria were isolated and identified such as Bacillus subtilis,(33.33), Bacillus pumulis (41.66), Bacillus licheniformis (25) were identified based on their colony biochemical characteristics.The isolates of the highest occurrences is bacillus pumilus. Agar well diffusion method was used to determine the antimicrobial activity against two pathogenic organisms, Escherichia coli, Staphylococcus aureus. Bacillus subtilis and Bacillus pumulis, gave a zone of inhibition Escherichia coli 0.6mm and 0.8mm after 24 hours of incubation also carbon sources of 5% and 10% sucrose, Glucose and soluble starch were used to check their different carbon sources on the isolates using the spectrophotometer.5% had the highest optimum growth of 1.400 for glucose 1.545 for sucrose,2.376 for soluble starch which can be added in maximum quantity to a medium to produce antibiotics.

Thesis Overview

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