1. Introduction
  1.1 Background
  1.2 Problem Statement
  1.3 Research Objectives
  1.4 Significance of the Study

2. Literature Review
  2.1 Animal Welfare during Transportation
  2.2 Factors Affecting Animal Stress during Transportation
  2.3 Handling Techniques and their Impact on Animal Welfare
  2.4 Gaps in Current Knowledge

3. Methodology
  3.1 Study Design
  3.2 Sample Selection
  3.3 Data Collection Methods
  3.4 Physiological Indicators of Stress
  3.5 Behavioral Indicators of Stress
  3.6 Statistical Analysis

4. Results
  4.1 Descriptive Statistics of Handling Techniques
  4.2 Physiological Responses of Animals to Different Handling Techniques
  4.3 Behavioral Responses of Animals to Different Handling Techniques
  4.4 Comparison of Handling Techniques

5. Discussion
  5.1 Interpretation of Results
  5.2 Comparison with Previous Studies
  5.3 Implications for Animal Welfare
  5.4 Limitations of the Study
  5.5 Recommendations for Future Research