Thesis Abstract

Abstract
Skeletal muscle development and function are crucial for overall human health and well-being. MicroRNAs (miRNAs) have emerged as key regulators of gene expression and play significant roles in various cellular processes, including muscle development and function. This thesis explores the role of miRNAs in regulating skeletal muscle development and function, aiming to provide a comprehensive understanding of the underlying mechanisms and potential implications for therapeutic interventions. The introductory chapter provides a background to the study, highlighting the importance of skeletal muscle development and function in human physiology. The problem statement underscores the gaps in current knowledge regarding the specific roles of miRNAs in regulating skeletal muscle development and function. The objectives of the study include investigating the expression patterns of miRNAs in skeletal muscle, elucidating their target genes, and exploring their functional significance in muscle development and function. The limitations and scope of the study are also outlined, along with the significance of the research in advancing our understanding of skeletal muscle biology. Chapter two presents a thorough literature review encompassing ten key areas related to miRNAs and skeletal muscle biology. This comprehensive review sets the stage for the subsequent chapters by summarizing existing knowledge and identifying gaps that warrant further investigation. Chapter three details the research methodology, including the experimental design, sample collection, RNA extraction, miRNA profiling, target gene prediction, functional assays, and data analysis. The methodology is designed to provide robust and reliable results to address the research objectives effectively. Chapter four presents a detailed discussion of the findings, including the expression profiles of miRNAs in skeletal muscle, their predicted target genes, and the functional implications of miRNA regulation in muscle development and function. The results are interpreted in the context of existing literature, highlighting the novel insights gained from this study. The concluding chapter summarizes the key findings of the research and their implications for understanding the role of miRNAs in regulating skeletal muscle development and function. The study contributes to the growing body of knowledge on miRNA-mediated gene regulation in skeletal muscle and provides a basis for future research in this field. Overall, this thesis enhances our understanding of the molecular mechanisms underlying skeletal muscle biology and opens new avenues for therapeutic interventions targeting miRNA pathways.

Thesis Overview

The project titled "The Role of MicroRNA in Regulating Skeletal Muscle Development and Function" aims to investigate the intricate mechanisms by which microRNAs influence the development and function of skeletal muscle. MicroRNAs are small non-coding RNA molecules that play crucial roles in post-transcriptional gene regulation by targeting messenger RNA (mRNA) for degradation or translational repression. In recent years, there has been growing interest in understanding the specific roles of microRNAs in skeletal muscle biology due to their potential implications in muscle development, regeneration, and disease. The research will delve into the current body of knowledge on microRNAs and their functions in skeletal muscle, with a focus on how they regulate key processes such as myogenesis, muscle growth, and maintenance of muscle mass. By conducting a comprehensive literature review, the study aims to identify the specific microRNAs that are known to be involved in these processes and elucidate the molecular mechanisms through which they exert their effects. Furthermore, the project will explore the potential therapeutic implications of targeting microRNAs for the treatment of muscle-related disorders and conditions. Understanding the role of microRNAs in skeletal muscle development and function may provide valuable insights into novel strategies for promoting muscle regeneration, enhancing muscle growth, and preventing muscle wasting in various pathological conditions. Through a combination of in vitro and in vivo experiments, the research will aim to validate the functional significance of specific microRNAs in skeletal muscle biology. By elucidating the regulatory networks controlled by microRNAs, the study seeks to contribute to the current understanding of muscle biology and provide a basis for the development of targeted interventions for muscle-related disorders. Overall, this project on "The Role of MicroRNA in Regulating Skeletal Muscle Development and Function" seeks to advance our knowledge of the molecular mechanisms underlying skeletal muscle biology and explore the therapeutic potential of targeting microRNAs for the treatment of muscle-related conditions. By shedding light on the complex interplay between microRNAs and skeletal muscle, this research has the potential to pave the way for innovative approaches in the field of muscle biology and regenerative medicine.